Thiols and disulfides: Difference between revisions

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Several possibilities are availbale for successful expression
Several possibilities are availbale for successful expression
*  Expression targeted to the periplasm. Several vectors for E.coli are available, e.g. containing the signal sequence of OmpA or for expression as MalE fusion.  
*  Expression targeted to the periplasm. Several vectors for E.coli are available, e.g. containing the signal sequence of OmpA or for expression as MalE fusion.  
*  Expression in yeast and secretion into the medium.
*  Expression in yeast and secretion into the medium.Here are also several systems available. Check out the current catalogues of molecular biology supply.
Here are also several systems available. Check out the current catalogues of molecular biology supply.
* Expression in E.coli strains with defects in maintaining the low redox potential of the cytoplasm. Strains carry mutations in thioredoxin reductase (trxB-, ADA494) or glutathione reductase (gor-) or both (Origami starin) and allow disulfide formation in the cytoplasm. Even expression of multiple Ig domains is possible in the Origami strain.  
* Expression in strains with defects in maintaining the low redoxpotential of the cytoplasm.




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