Difference between revisions of "Thiols and disulfides"

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(New page: Expression of proteins containing disulfides Analysis of free thiols Analysis of disulfides)
 
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Expression of proteins containing disulfides
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Expression of proteins containing disulfides (editing in progress, April 23, 2008)
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Expression of proteins containing disulfides in the native state can make a lot of trouble when expressed using standard vectors and strains for cytoplasmic expression.
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Several possibilities are availbale for successful expression
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1. Expression targetetd to the periplasm
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2. Expression in yeast and secretion into the medium
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3. Expression in strains with defects in maintaining the low redxpotential of the cytplasm
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 +
 
  
 
Analysis of free thiols
 
Analysis of free thiols
  
 
Analysis of disulfides
 
Analysis of disulfides

Revision as of 12:37, 24 April 2008

Expression of proteins containing disulfides (editing in progress, April 23, 2008)

Expression of proteins containing disulfides in the native state can make a lot of trouble when expressed using standard vectors and strains for cytoplasmic expression.

Several possibilities are availbale for successful expression 1. Expression targetetd to the periplasm 2. Expression in yeast and secretion into the medium 3. Expression in strains with defects in maintaining the low redxpotential of the cytplasm


Analysis of free thiols

Analysis of disulfides