The Paris protocol ...

From CCP4 wiki
Revision as of 21:25, 25 July 2009 by Mmiller (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search

This work is in progress, based on pET21a inserting between NdeI and NotI sites.

The protocol being used is essentially as in Elledge, 2007, with RecA.

See supplementary protcol #2 file - via CNRS Direct Nature URL from

Li M.Z. & Elledge S. J. Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nature Methods 4, 251 - 256 (2007) doi:10.1038/nmeth1010