Difference between revisions of "The Paris protocol ..."

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This work is in progress, based on pET21a inserting between NdeI and NotI sites.
 
This work is in progress, based on pET21a inserting between NdeI and NotI sites.
  
The protocol being used is essentially as in Elledge, 2007, with RecA. [http://www.nature.com.gate1.inist.fr/nmeth/journal/v4/n3/extref/nmeth1010_S6.pdf]
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The protocol being used is essentially as in Elledge, 2007, with RecA.  
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See supplementary protcol #2 file - [http://www.nature.com.gate1.inist.fr/nmeth/journal/v4/n3/extref/nmeth1010_S6.pdf via CNRS] [http://www.nature.com/nmeth/journal/v4/n3/extref/nmeth1010_S6.pdf Direct Nature URL] from
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Li M.Z. & Elledge S. J. Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. ''Nature Methods'' '''4''', 251 - 256 (2007) [http://dx.doi.org/10.1038/nmeth1010 doi:10.1038/nmeth1010]

Latest revision as of 21:25, 25 July 2009

This work is in progress, based on pET21a inserting between NdeI and NotI sites.

The protocol being used is essentially as in Elledge, 2007, with RecA.

See supplementary protcol #2 file - via CNRS Direct Nature URL from

Li M.Z. & Elledge S. J. Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nature Methods 4, 251 - 256 (2007) doi:10.1038/nmeth1010