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Sticky crystals: Difference between revisions
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‘IN-PLATE’ DIFFRACTION EXPERIMENTS | ‘IN-PLATE’ DIFFRACTION EXPERIMENTS | ||
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Beamline FIP-BM30A at the ESRF (contact Jean-Luc Ferrer) has a setup that allows crystal testing, even data collection, while the crystal remains in the crystallization drop. | Beamline FIP-BM30A at the ESRF (contact Jean-Luc Ferrer) has a setup that allows crystal testing, even data collection, while the crystal remains in the crystallization drop (see movie at [http://www.natx-ray.com/products/G-Rob_2D_movie.html]). A lab system is commercialized by NatX-ray [http://www.natx-ray.com/products/G-Rob_2D.html]. | ||
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PIPETTING techniques | PIPETTING techniques | ||
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Add a drop of 10ul mother liquor to the crystallization drop. Sometimes, the crystals attached to the plastic would float to the surface. If not, take another 10ul and pipet it in and out the drop as close as possible to the bottom. | Add a drop of 10ul mother liquor to the crystallization drop. Sometimes, the crystals attached to the plastic would float to the surface. If not, take another 10ul and pipet it in and out the drop as close as possible to the bottom. This might dislodge additional crystals from the plastic. | ||