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Sticky crystals: Difference between revisions
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Crystal harvesting out of native crystallization drops (hanging- and/or sitting-drop geometries) can sometimes be severely hampered by the propensity of crystals to stick to their crystallization surface. Here are some tips on how to approach the problem: | Crystal harvesting out of native crystallization drops (hanging- and/or sitting-drop geometries) can sometimes be severely hampered by the propensity of crystals to stick (sometimes very tightly) to their crystallization surface. Here are some tips on how to approach the problem: | ||
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COAT THE CRYSTALLIZATION SURFACE WITH SILICON | COAT THE CRYSTALLIZATION SURFACE WITH SILICON | ||
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You can try various siliconizing fluids such as AquaSil (Hampton Research). Such a product will not melt the plastic of the crystallization plate as opposed to | You can try various siliconizing fluids such as AquaSil (Hampton Research). Such a product will not melt the plastic of the crystallization plate as opposed to e.g. Repelcote. | ||
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‘IN-PLATE’ DIFFRACTION EXPERIMENTS | ‘IN-PLATE’ DIFFRACTION EXPERIMENTS | ||
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Beamline FIP-BM30A at the ESRF (contact Jean-Luc Ferrer) has a setup that allows crystal testing, even data collection, while the crystal remains in the crystallization drop. | Beamline FIP-BM30A at the ESRF (contact Jean-Luc Ferrer) has a setup that allows crystal testing, even data collection, while the crystal remains in the crystallization drop (see movie at [http://www.natx-ray.com/products/G-Rob_2D_movie.html]). A lab system is commercialized by NatX-ray [http://www.natx-ray.com/products/G-Rob_2D.html]. | ||
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PIPETTING techniques | PIPETTING techniques | ||
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Add a drop of 10ul mother liquor to the crystallization drop. Sometimes, the crystals attached to the plastic would float to the surface. If not, take another 10ul and pipet it in and out the drop as close as possible to the bottom. | Add a drop of 10ul mother liquor to the crystallization drop. Sometimes, the crystals attached to the plastic would float to the surface. If not, take another 10ul and pipet it in and out the drop as close as possible to the bottom. This might dislodge additional crystals from the plastic. | ||