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Crystal growth: Tips and Tricks: Difference between revisions
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→Reducing the mosaicity
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==Improving crystals== | ==Improving crystals== | ||
===Reducing the mosaicity=== | ===Reducing the mosaicity=== | ||
Things to try in the order crystallization-related / cryo-related / geometry-related: | |||
# change precipitant, pH, temperature; try additives | |||
# try co-crystallization with glycerol (2%, 5% or 10%). | # try co-crystallization with glycerol (2%, 5% or 10%). | ||
# incubate your crystal longer in your cryo/stabilization solution. | |||
# optimize the cryo conditions | |||
# anneal (freeze/thaw) the crystal - '''DISCLAIMER''': your mosaicity can go up as well as down with annealing | |||
# the mosaicity may be anisotropic, so try to orient the crystal in the loop such that you can shoot along a different axis | # the mosaicity may be anisotropic, so try to orient the crystal in the loop such that you can shoot along a different axis | ||
# | # try smaller crystals (often growth faults lead to end of crystal growth) | ||
# use a synchrotron beam (as it is less divergent than the usual home source, and the apparent mosaicity is the sum of the crystal mosaicity and the crossfire) | # use a synchrotron beam (as it is less divergent than the usual home source, and the apparent mosaicity is the sum of the crystal mosaicity and the crossfire) | ||
# shoot at parts of the crystal to find if there are good and bad parts (this requires a small beam) | # shoot at parts of the crystal to find if there are good and bad parts (this requires a small beam) | ||
# look at Elspeth Garman's papers, and/or check her talks at RapiData workshops (anybody with a link??) | # look at Elspeth Garman's papers, and/or check her talks at RapiData workshops (anybody with a link??) | ||