Crystal growth: Tips and Tricks: Difference between revisions

# incubate your crystal longer in your cryo/stabilization solution. This helps sometimes.  

# try co-crystallization with glycerol (2%, 5% or 10%).

# the mosaicity may be anisotropic, so try to orient the crystal in the loop such that you can shoot along a different axis

# change precipitant, pH, temperature, add additives

# optimize cryo conditions

# use a synchrotron beam (as it is less divergent than the usual home source, and the apparent mosaicity is the sum of the crystal mosaicity and the crossfire)

# try smaller crystals (often growth faults lead to end of crystal growth)

# shoot at parts of the crystal to find if there are good and bad parts (this requires a small beam)

# anneal (freeze/thaw) the crystal - '''DISCLAIMER''': your mosaicity can go up as well as down with annealing