Thiols and disulfides: Difference between revisions

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(New page: Expression of proteins containing disulfides Analysis of free thiols Analysis of disulfides)
 
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Expression of proteins containing disulfides
Expression of proteins containing disulfides (editing in progress, April 23, 2008)
 
Expression of proteins containing disulfides in the native state can make a lot of trouble when expressed using standard vectors and strains for cytoplasmic expression.
 
Several possibilities are availbale for successful expression
1. Expression targetetd to the periplasm
2. Expression in yeast and secretion into the medium
3. Expression in strains with defects in maintaining the low redxpotential of the cytplasm
 
 


Analysis of free thiols
Analysis of free thiols


Analysis of disulfides
Analysis of disulfides
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