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=== help, my protein has high B-factors! === | === help, my protein has high B-factors! === | ||
This is also a FAQ on CCP4BB. The answer is: there's probably nothing wrong with it. If your crystals diffract to 3 A at a synchrotron, then the average B-factor should most likely be on the order of 100 A^2. If your crystals diffract to 2 A, then the average B-factor is most likely on the order of 40 A^2 or so. ( | This is also a FAQ on CCP4BB. The answer is: there's probably nothing wrong with it. If your crystals diffract to 3 A at a synchrotron, then the average B-factor should most likely be on the order of 100 A^2. If your crystals diffract to 2 A, then the average B-factor is most likely on the order of 40 A^2 or so. Use B. Rupp's calculator ([http://www.ruppweb.org/new_comp/scattering_factors.htm]) to find out the dependance of scattering power on B-factor. | ||
=== R_free much higher than R === | === R_free much higher than R === | ||