Changes

From CCP4 wiki
Jump to navigationJump to search
no edit summary
Line 17: Line 17:  
3.  Set your screens at different temperature.  25 and 4 degrees are the most popular options.  If you completely lost hope for your original screens, just transfer them to the cold room.  Protein solubility will change and you may get crystals.
 
3.  Set your screens at different temperature.  25 and 4 degrees are the most popular options.  If you completely lost hope for your original screens, just transfer them to the cold room.  Protein solubility will change and you may get crystals.
   −
4.  Add a purification step. Purity can be critical to protein crystallization and the purity
+
4.  Add a purification step. Purity can be critical to protein crystallization and the purity judged from SDS-PAGE or even gel filtration can be misleading.
    judged from SDS-PAGE or even gel filtration can be misleading.
      
5.  Modify your protein.  See [[Modifying_the_protein_to_crystallize_better]]
 
5.  Modify your protein.  See [[Modifying_the_protein_to_crystallize_better]]
25

edits

Cookies help us deliver our services. By using our services, you agree to our use of cookies.

Navigation menu