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Inserted item 4: additional purification step.
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3.  Set your screens at different temperature.  25 and 4 degrees are the most popular options.  If you completely lost hope for your original screens, just transfer them to the cold room.  Protein solubility will change and you may get crystals.
 
3.  Set your screens at different temperature.  25 and 4 degrees are the most popular options.  If you completely lost hope for your original screens, just transfer them to the cold room.  Protein solubility will change and you may get crystals.
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4.  Modify your protein.  See [[Modifying_the_protein_to_crystallize_better]]
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4.  Add a purification step. Purity can be critical to protein crystallization and the purity
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    judged from SDS-PAGE or even gel filtration can be misleading.
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5.  Modify your protein.  See [[Modifying_the_protein_to_crystallize_better]]
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