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Modifying the protein to crystallize better: Difference between revisions
Modifying the protein to crystallize better (edit)
Revision as of 16:01, 13 March 2008
, 13 March 2008Editorial changes - please dont use xxx unless you want to create a page with that name!
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== | == Introduction== | ||
There are a number of methods which can be used to modify the crystallization behavior of proteins. | There are a number of methods which can be used to modify the crystallization behavior of proteins. | ||
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== | === 1. Genetic/Mutagenesis Approach=== | ||
This method, also called 'surface entropy-reduction mutagenesis', involves mutation of sidechains on the surface of proteins, in order to reduce the entropic cost of forming ordered intermolecular crystal contacts and thus enhance the crystallizability of proteins. | This method, also called 'surface entropy-reduction mutagenesis', involves mutation of sidechains on the surface of proteins, in order to reduce the entropic cost of forming ordered intermolecular crystal contacts and thus enhance the crystallizability of proteins. | ||
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== | === Chemical Modification=== | ||
Another method for enhancing the crystallizability of proteins involves chemical modification of specific sidechains. | Another method for enhancing the crystallizability of proteins involves chemical modification of specific sidechains. | ||
Several methods are available: | Several methods are available: | ||
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A recent study (Walter et al. [2006] "Lysine Methylation as a Routine Rescue Strategy for Protein Crystallization" Structure 14:1617–1622) demonstrated that, out of 10 non-crystallizable proteins, | A recent study (Walter et al. [2006] "Lysine Methylation as a Routine Rescue Strategy for Protein Crystallization" Structure 14:1617–1622) demonstrated that, out of 10 non-crystallizable proteins, | ||
4 proteins became crystallisable after reductive methylation. | 4 proteins became crystallisable after reductive methylation. | ||
Here is a protocol for [[Lysine Methylation]]. | Here is a protocol for [[Lysine Methylation]]. | ||