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A recent study (Walter et al. [2006] "Lysine Methylation as a Routine Rescue Strategy for Protein Crystallization" Structure 14:1617–1622) demonstrated that, out of 10 non-crystallizable proteins,  
 
A recent study (Walter et al. [2006] "Lysine Methylation as a Routine Rescue Strategy for Protein Crystallization" Structure 14:1617–1622) demonstrated that, out of 10 non-crystallizable proteins,  
 
4 proteins became crystallisable after reductive methylation.
 
4 proteins became crystallisable after reductive methylation.
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Here is a protocol for [[Lysine Methylation]].
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Here is a [[Lysine_Methylation|protocol]].
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2.2. Modification of cysteine residues  
 
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2.2. Modification of cyteine residues  
      
This method involves the carboxymethylation -- under reducing conditions -- of single cysteine residues. This effectively neutralizes the cysteine residues (some of which are chemically reactive), increases the overall solubility of the protein and can help prevent aggregation and denaturation problems.
 
This method involves the carboxymethylation -- under reducing conditions -- of single cysteine residues. This effectively neutralizes the cysteine residues (some of which are chemically reactive), increases the overall solubility of the protein and can help prevent aggregation and denaturation problems.
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