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*  8. Dry pellet on a heat block at 95°C for 5-10 minutes to drive off acetone.
 
*  8. Dry pellet on a heat block at 95°C for 5-10 minutes to drive off acetone.
 
*  9. Add loading buffer to sample and boil before loading on gel.
 
*  9. Add loading buffer to sample and boil before loading on gel.
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== Protein precipitates? ==
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To increase solubility, follow Golovanov et al. 2004. In short,
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* buy L-Arg and L-Glu
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* put L-Arginine powder into water, start dissolving it, then add L-Glu powder, stir; add water to adjust volume as required
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* max. solubility is 1 M. The pH of a 1M stock solution will be around 6.4 or close to neutral
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* add stock solution (50mM) to your protein
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