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7. Other useful tips?
 
7. Other useful tips?
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Protein DNA crystals can be very fragile so good idea to screen with cryoprotectant in the screen to save manipulations
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* Protein DNA crystals can be very fragile so good idea to screen with cryoprotectant in the screen to save manipulations
Test crystals with a gel to see if DNA is present
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* Test crystals with a gel to see if DNA is present
Use of SAXS to get molecular envelop of complex
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* Use of SAXS to get molecular envelop of complex
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* general guidelines to prepare protein/DNA complexes suitable for crystallization trials: [http://dx.doi.org/10.1007/978-1-59745-209-0_11 Hollis T (2007) Crystallization of protein-DNA complexes. Methods Mol. Biol. 363: 225–237]
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* design and composition of a specialized screen for protein/DNA complexes: [http://dx.doi.org/10.1107/S1744309112025316 Pryor EE Jr, Wozniak DJ & Hollis T (2012) Crystallization of Pseudomonas aeruginosa AmrZ protein: development of a comprehensive method for obtaining and optimization of protein-DNA crystals. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 68: 985–993]
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Several references below:
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== Some references: ==
    
Schultz, S. C., Shields, G. C. & Steitz, T. A. (1990).
 
Schultz, S. C., Shields, G. C. & Steitz, T. A. (1990).
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