1,315
edits
Changes
From CCP4 wiki
Jump to navigationJump to searchCrystal growth: Protein-DNA complexes (edit)
Revision as of 08:54, 9 September 2015
, 08:54, 9 September 2015no edit summary
7. Other useful tips?
7. Other useful tips?
Protein DNA crystals can be very fragile so good idea to screen with cryoprotectant in the screen to save manipulations
* Protein DNA crystals can be very fragile so good idea to screen with cryoprotectant in the screen to save manipulations
Test crystals with a gel to see if DNA is present
* Test crystals with a gel to see if DNA is present
Use of SAXS to get molecular envelop of complex
* Use of SAXS to get molecular envelop of complex
* general guidelines to prepare protein/DNA complexes suitable for crystallization trials: [http://dx.doi.org/10.1007/978-1-59745-209-0_11 Hollis T (2007) Crystallization of protein-DNA complexes. Methods Mol. Biol. 363: 225–237]
* design and composition of a specialized screen for protein/DNA complexes: [http://dx.doi.org/10.1107/S1744309112025316 Pryor EE Jr, Wozniak DJ & Hollis T (2012) Crystallization of Pseudomonas aeruginosa AmrZ protein: development of a comprehensive method for obtaining and optimization of protein-DNA crystals. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 68: 985–993]
== Some references: ==
Schultz, S. C., Shields, G. C. & Steitz, T. A. (1990).
Schultz, S. C., Shields, G. C. & Steitz, T. A. (1990).
