Changes

No change in size ,  16:34, 12 February 2008
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==Mammalian cells==
 
==Mammalian cells==
  −
===Chinese hamster ovary (CHO) cultured cells===
      
===Human Embryonic Kidney (HEK) 293 cultured cells ===
 
===Human Embryonic Kidney (HEK) 293 cultured cells ===
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Quite easy to scale up the volume still using attached cells, in special plastic bottles that present a large surface area: thats most useful especially for secreted proteins. There are two main ways to express your gene in HEK293 cells:
 
Quite easy to scale up the volume still using attached cells, in special plastic bottles that present a large surface area: thats most useful especially for secreted proteins. There are two main ways to express your gene in HEK293 cells:
 
Producing intracellular proteins in HEK293 cells requires to grow the cells in suspension which can be a bit more of an art and requires a CO2 shaking incubator.
 
Producing intracellular proteins in HEK293 cells requires to grow the cells in suspension which can be a bit more of an art and requires a CO2 shaking incubator.
 +
 +
===Chinese hamster ovary (CHO) cultured cells===
    
=Other systems=
 
=Other systems=
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