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Expression systems: Difference between revisions
→Mammalian cells
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==Mammalian cells== | ==Mammalian cells== | ||
===Human Embryonic Kidney (HEK) 293 cultured cells === | ===Human Embryonic Kidney (HEK) 293 cultured cells === | ||
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Quite easy to scale up the volume still using attached cells, in special plastic bottles that present a large surface area: thats most useful especially for secreted proteins. There are two main ways to express your gene in HEK293 cells: | Quite easy to scale up the volume still using attached cells, in special plastic bottles that present a large surface area: thats most useful especially for secreted proteins. There are two main ways to express your gene in HEK293 cells: | ||
Producing intracellular proteins in HEK293 cells requires to grow the cells in suspension which can be a bit more of an art and requires a CO2 shaking incubator. | Producing intracellular proteins in HEK293 cells requires to grow the cells in suspension which can be a bit more of an art and requires a CO2 shaking incubator. | ||
===Chinese hamster ovary (CHO) cultured cells=== | |||
=Other systems= | =Other systems= | ||