Expression systems: Difference between revisions

Expression systems (edit)

Revision as of 18:03, 12 February 2008

24 bytes added , 12 February 2008

Changed to a more of a chpater format to encourage people to post more details !?!?

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-=Bacteria=
+=Mainstream systems=
-   ''Escherichia coli''
+==''Escherichia coli''==
-   ''Bacillus subtilis''
+===Rosetta cells ===
-   ''Bacillus megaterium''
-   ''Pseudomonas fluorescens''
-=Protozoa=
+If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well.  Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem.  For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest".
-   ''Leishmania tarentolae''
+==Fungi==
+===''Pichia pastoris''===
+==Insect cells==
+===Baculovirus-mediated expression in ''Spodoptera frugiperd'' and ''Trichoplusia ni'' cells===
+==Mammalian cells==
+===Chinese hamster ovary (CHO) cultured cells===
+===Human Embryonic Kidney (HEK) 293 cultured cells ===
+*[http://www.ncbi.nlm.nih.gov/pubmed/17001101?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Transient transfection].
+*[http://www.invitrogen.com/content.cfm?pageid=3375 Flp-In for stable cell lines ].
+=Other systems=
+==Bacteria==
+===''Bacillus subtilis''===
+===''Bacillus megaterium''===
+===''Pseudomonas fluorescens''===
+==Protozoa==
+===''Leishmania tarentolae''===
-=Fungi=
+==Fungi==
-   ''Pichia pastoris''
-   ''Saccharomyces cerevisiae''
-   ''Schizosaccharomyces pombe''
-   ''Hansenula polymorpha''
-   Filamentous fungi
-=Insect cells=
+===''Saccharomyces cerevisiae''===
-   Baculovirus-mediated expression in "Spodoptera frugiperda" and "Trichoplusia ni" cells
+===''Schizosaccharomyces pombe''===
+===''Hansenula polymorpha''===
+===''Filamentous fungi''===
-=Mammalian cells=
-   Chinese hamster ovary (CHO) cultured cells
-   Human Embryonic Kidney (HEK) 293 cultured cells [http://www.ncbi.nlm.nih.gov/pubmed/17001101?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum (Transient transfection)].
-   Human Embryonic Kidney (HEK) 293 cultured cells [http://www.invitrogen.com/content.cfm?pageid=3375 (Flp-In System)].
 =Plant cells=
-   Algae
-   Chloroplasts
- ''Nicotiana tabacum''
-= Try Rosetta cells =
+==Algae==
-If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well.  Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem.  For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest".
+Chloroplasts
+''Nicotiana tabacum''
---[[User:Zelig ff|Artem]] 02:43, 11 February 2008 (CET)