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24 bytes added ,  16:03, 12 February 2008
Changed to a more of a chpater format to encourage people to post more details !?!?
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=Bacteria=
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=Mainstream systems=
   −
  ''Escherichia coli''
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==''Escherichia coli''==
  ''Bacillus subtilis''
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===Rosetta cells ===
  ''Bacillus megaterium''
  −
  ''Pseudomonas fluorescens''
     −
=Protozoa=
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If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well.  Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem.  For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest".
  ''Leishmania tarentolae''
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 +
==Fungi==
 +
 
 +
===''Pichia pastoris''===
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 +
 
 +
==Insect cells==
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 +
===Baculovirus-mediated expression in ''Spodoptera frugiperd'' and ''Trichoplusia ni'' cells===
 +
 
 +
==Mammalian cells==
 +
 
 +
===Chinese hamster ovary (CHO) cultured cells===
 +
 
 +
===Human Embryonic Kidney (HEK) 293 cultured cells ===
 +
*[http://www.ncbi.nlm.nih.gov/pubmed/17001101?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Transient transfection].
 +
 
 +
*[http://www.invitrogen.com/content.cfm?pageid=3375 Flp-In for stable cell lines ].
 +
 
 +
=Other systems=
 +
 
 +
==Bacteria==
 +
 
 +
===''Bacillus subtilis''===
 +
 
 +
===''Bacillus megaterium''===
 +
 
 +
===''Pseudomonas fluorescens''===
 +
 
 +
==Protozoa==
 +
 
 +
===''Leishmania tarentolae''===
   −
=Fungi=
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==Fungi==
  ''Pichia pastoris''
  −
  ''Saccharomyces cerevisiae''
  −
  ''Schizosaccharomyces pombe''
  −
  ''Hansenula polymorpha''
  −
  Filamentous fungi
     −
=Insect cells=
+
===''Saccharomyces cerevisiae''===
  Baculovirus-mediated expression in "Spodoptera frugiperda" and "Trichoplusia ni" cells
+
 
 +
===''Schizosaccharomyces pombe''===
 +
 
 +
===''Hansenula polymorpha''===
 +
 
 +
===''Filamentous fungi''===
   −
=Mammalian cells=
     −
  Chinese hamster ovary (CHO) cultured cells
  −
  Human Embryonic Kidney (HEK) 293 cultured cells [http://www.ncbi.nlm.nih.gov/pubmed/17001101?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum (Transient transfection)].
  −
  Human Embryonic Kidney (HEK) 293 cultured cells [http://www.invitrogen.com/content.cfm?pageid=3375 (Flp-In System)].
      
=Plant cells=
 
=Plant cells=
  Algae
  −
  Chloroplasts
  −
''Nicotiana tabacum''
     −
= Try Rosetta cells =
+
==Algae==
   −
If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well.  Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem.  For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest".
+
Chloroplasts
   −
 
+
''Nicotiana tabacum''
--[[User:Zelig ff|Artem]] 02:43, 11 February 2008 (CET)
 
84

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