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2 bytes removed ,  08:35, 11 February 2008
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   Algae
 
   Algae
 
   Chloroplasts
 
   Chloroplasts
 +
''Nicotiana tabacum''
   −
== Try Rosetta cells ==
+
= Try Rosetta cells =
    
If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well.  Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem.  For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest".
 
If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well.  Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem.  For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest".
   −
  ''Nicotiana tabacum''
+
 
 
   
--[[User:Zelig ff|Artem]] 02:43, 11 February 2008 (CET)
 
--[[User:Zelig ff|Artem]] 02:43, 11 February 2008 (CET)
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