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Expression of SeMet labeled proteins: Difference between revisions
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= bacterial expression | = bacterial expression = | ||
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= expression | = expression in other systems = | ||
* mammalian cells: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242577/pdf/2008.pdf mentions several mammalian cell lines, including CHO, HEK239, and COS. Apparently, Lec8 have been used, too. | * mammalian cells: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242577/pdf/2008.pdf mentions several mammalian cell lines, including CHO, HEK239, and COS. Apparently, Lec8 have been used, too. | ||
* Yeast | * Yeast | ||
* stable S2 insect cells | * stable S2 insect cells | ||
* baculovirus expression system | * baculovirus expression system | ||
* CHO LecR cells, early reference (1994) from Wayne Hendrickson's lab (PMID: 7922031) | |||
* probably the easiest labelling method for HEK293 cells can be found in PMID: 17001101. | |||