Modifying the protein to crystallize better: Difference between revisions

Modifying the protein to crystallize better (edit)

234 bytes added , 10 February 2008

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Revision as of 13:50, 10 February 2008 (edit) Tassos (talk \| contribs) m (→‎[[Lysine Methylation]]) ← Older edit		Revision as of 13:51, 10 February 2008 (edit) (undo) Tassos (talk \| contribs) m (→‎[[Lysine Methylation]]) Newer edit →
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	== [[Lysine Methylation]]==		== [[Lysine Methylation]]==
	This protocol is an NKI local adaptation based on the article by Grimes and co-workers[http://www.ncbi.nlm.nih.gov/pubmed/17098187?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum]		This protocol is an NKI local adaptation based on the article by Grimes and co-workers[http://www.ncbi.nlm.nih.gov/pubmed/17098187?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum]. The idea is to change the charge properties post-purification, modify the solubility of the protein (hence the phase diagram) and get crystals! Its a quick and easy thing to try especially for proteins that appear to be too soluble!