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This work is in progress, based on pET21a inserting between NdeI and NotI sites.
 
This work is in progress, based on pET21a inserting between NdeI and NotI sites.
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The protocol being used is essentially as in Elledge, 2007, with RecA. [http://www.nature.com.gate1.inist.fr/nmeth/journal/v4/n3/extref/nmeth1010_S6.pdf]
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The protocol being used is essentially as in Elledge, 2007, with RecA.  
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See supplementary protcol #2 file - [http://www.nature.com.gate1.inist.fr/nmeth/journal/v4/n3/extref/nmeth1010_S6.pdf via CNRS] [http://www.nature.com/nmeth/journal/v4/n3/extref/nmeth1010_S6.pdf Direct Nature URL] from
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Li M.Z. & Elledge S. J. Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. ''Nature Methods'' '''4''', 251 - 256 (2007) [http://dx.doi.org/10.1038/nmeth1010 doi:10.1038/nmeth1010]
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