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263 bytes removed ,  09:37, 15 February 2009
→‎Reducing the mosaicity: links and rearrangement vs cryo articles
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==Improving crystals==
 
==Improving crystals==
 
===Reducing the mosaicity===
 
===Reducing the mosaicity===
Things to try in the order crystallization-related / cryo-related / geometry-related:
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# change precipitant, pH, temperature; try additives
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Things to try concerning crystallization - see also [[Improving crystal quality]].
# try co-crystallization with glycerol (2%, 5% or 10%).
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For cryo question see [[cryo]] and [[Data collection: Tips and Tricks]].
# incubate your crystal longer in your cryo/stabilization solution.
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# optimize the cryo conditions
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* change precipitant, pH, temperature; try additives
# anneal (freeze/thaw) the crystal - '''DISCLAIMER''': your mosaicity can go up as well as down with annealing
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* try co-crystallization with glycerol (2%, 5% or 10%).
# the mosaicity may be anisotropic, so try to orient the crystal in the loop such that you can shoot along a different axis
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* incubate your crystal longer in your cryo/stabilization solution.
# try smaller crystals (often growth faults lead to end of crystal growth)
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* optimize the cryo conditions
# use a synchrotron beam (as it is less divergent than the usual home source, and the apparent mosaicity is the sum of the crystal mosaicity and the crossfire)
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* anneal (freeze/thaw) the crystal - '''DISCLAIMER''': your mosaicity can go up as well as down with annealing
# shoot at parts of the crystal to find if there are good and bad parts (this requires a small beam)
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* the mosaicity may be anisotropic, so try to orient the crystal in the loop such that you can shoot along a different axis
# look at Elspeth Garman's papers, and/or check her talks at RapiData workshops (anybody with a link??)
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* look at Elspeth Garman's papers, and/or check her talks at RapiData workshops (anybody with a link??)
    
==References==
 
==References==
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