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→‎Data collection: separate X-ray generators and detectors
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== Crystal Characterization ==
 
== Crystal Characterization ==
 
* [[Birefringence]]
 
* [[Birefringence]]
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* [[solvent content]]
    
== Crystal Imperfections ==
 
== Crystal Imperfections ==
 
* [[Diffuse Scattering]]
 
* [[Diffuse Scattering]]
 
* [[Incommensurate Lattices]]
 
* [[Incommensurate Lattices]]
 +
* [[Examples of diffraction patterns showing particular features]]
    
== Crystal Growth ==
 
== Crystal Growth ==
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* [[Conditions prone to salt crystallization]]
 
* [[Conditions prone to salt crystallization]]
 
* [[Biophysical methods]] for evaluation of crystallization tendency
 
* [[Biophysical methods]] for evaluation of crystallization tendency
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* [[Improving crystal quality]]
 
* [[Robots for crystallization setup]]
 
* [[Robots for crystallization setup]]
 
* [[Visualization of crystal growth]]
 
* [[Visualization of crystal growth]]
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* [[Robots for crystallization monitoring]]
 
* [[Robots for crystallization monitoring]]
 
* [[Crystal growth: Tips and Tricks]]
 
* [[Crystal growth: Tips and Tricks]]
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* [[Crystal growth: Protein-DNA complexes]]
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* [[BMCD|Biomolecular crystallization database]]
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* Prediction of protein crystallizability: [http://ffas.burnham.org/XtalPred-cgi/xtal.pl XtalPred]
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* crystallization of soluble proteins (see also [[Improving crystal quality]])
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* crystallization of membrane proteins (see also [[Improving crystal quality#Improving diffraction quality of membrane protein crystals]])
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* relative humidity calculations of salt/PEG solutions - can also be used to calculate equivalent/matching osmotic pressure: http://go.esrf.eu/RH
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== Crystal handling ==
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* [[Cryo]]
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* [[Sticky crystals]]
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* [[Soaking with heavy atoms]]
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* [[Microseeding]]
    
== Data collection ==
 
== Data collection ==
* [[X-ray generators and detectors]] (experiences)
+
* [[X-ray generators]]  
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* [[Detectors]]
 
* [[Synchrotrons]]
 
* [[Synchrotrons]]
* [[Cryo]]
   
* [[Strategies]]
 
* [[Strategies]]
 
* [[Data quality]]
 
* [[Data quality]]
 
* [[Radiation damage]]
 
* [[Radiation damage]]
 +
* [[some ways to calculate the radiation dose that a crystal has absorbed]]
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* [[Comparison of home source and synchrotron properties]]
 
* [[Data collection: Tips and Tricks]]
 
* [[Data collection: Tips and Tricks]]
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* [[Storage of diffraction data]]
    
== References ==
 
== References ==
   −
* crystallization of soluble proteins
  −
  −
* crystallization of membrane proteins
   
# Wiener MC (2004) A pedestrian guide to membrane protein crystallization. Methods 34(3), 364-372.
 
# Wiener MC (2004) A pedestrian guide to membrane protein crystallization. Methods 34(3), 364-372.
 
# Loll PJ (2003) Membrane protein structural biology: the high throughput challenge. J. Struct. Biol. 142(1), 144-153.
 
# Loll PJ (2003) Membrane protein structural biology: the high throughput challenge. J. Struct. Biol. 142(1), 144-153.
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# Macromolecular Crystallography Protocols, Volume 1: Preparation and Crystallization of Macromolecules. Doublie S (Ed.), Totowa, NJ: Humana Press. (2007)
 
# Macromolecular Crystallography Protocols, Volume 1: Preparation and Crystallization of Macromolecules. Doublie S (Ed.), Totowa, NJ: Humana Press. (2007)
 
# Structural Biology of Membrane Proteins. Grisshammer R, & Buchanan SK (Eds.), Cambridge, UK: Royal Society of Chemistry. (2006)
 
# Structural Biology of Membrane Proteins. Grisshammer R, & Buchanan SK (Eds.), Cambridge, UK: Royal Society of Chemistry. (2006)
 +
# Macromolecular Crystallisation and Crystal Perfection. N E Chayen, J R Helliwell and E H Snell OUP 2010 [http://www.oxfordscholarship.com/oso/public/content/physics/9780199213252/toc.html]
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