Studier FW (2005) Protein production by auto-induction in high density shaking cultures. Protein Expr. Purif. 41, 207-234
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Online:
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Two protocols for semet labelling as of 11/03/2008:
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http://www.doe-mbi.ucla.edu/local/protocols
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Another non-auxotroph protocol is copied below, courtesy of G. Fritz. Fritz says this recipe could probably be made in one vessel for immediate use, except for the aromatic aa's which might dissolve better at basic pH: