23 February 2009
- 11:4311:43, 23 February 2009 diff hist −4 m Experimental phasing No edit summary
- 11:4211:42, 23 February 2009 diff hist +435 Experimental phasing Add CNS (shame on me for forgetting) and minor changes
12 February 2009
- 11:3111:31, 12 February 2009 diff hist +3,144 Experimental phasing a first try
30 January 2009
- 00:1500:15, 30 January 2009 diff hist +168 N Mapping sequence alignment to a structure New page: [http://consurf.tau.ac.il/ ConSurf] web server [http://www.mcgnmr.ca/ProtSkin/intro/ ProtSkin] web server [http://www.ncbi.nlm.nih.gov/pubmed/11524371 AL2CO] software
- 00:1000:10, 30 January 2009 diff hist +48 Bioinformatics No edit summary
2 September 2008
- 15:1115:11, 2 September 2008 diff hist +7,076 N LIC cloning New page: '''LIC-cloning into the pET-NKI LIC vectors''' [contributed by Vangelis Christodoulou, NKI] This protocol is based on the "version 2" LIC system designed by Vangelis. '''DAY -20 to -10...
- 15:0915:09, 2 September 2008 diff hist +18 m Expression and Purification No edit summary
19 June 2008
- 13:5813:58, 19 June 2008 diff hist +1,924 N The Milan protocol ... New page: =SLIC Sub-cloning using T4 DNA polymerase treated insert= * Digest 3 μg vector with two restriction enzymes. Gel-purify the vector and isolate the DNA using Qiagen extraction kit. Quanti...
18 June 2008
- 09:0709:07, 18 June 2008 diff hist +109 Test set cosmetics and wiki links
- 09:0509:05, 18 June 2008 diff hist +34 Test set →How precise is the estimate of Rfree for a certain number of test set reflections?
29 March 2008
- 00:4100:41, 29 March 2008 diff hist +67 Structure based sequence alignment No edit summary
22 March 2008
- 20:3120:31, 22 March 2008 diff hist −37 m Tags changed appearance from code to tabbed list
- 20:2920:29, 22 March 2008 diff hist +7 m Positions Same as for Events
- 20:2820:28, 22 March 2008 diff hist +5 m Current events No edit summary
- 20:2720:27, 22 March 2008 diff hist 0 m Current events the instructions go to the end ?
- 10:1410:14, 22 March 2008 diff hist +690 ARP/wARP No edit summary current
17 March 2008
- 20:2420:24, 17 March 2008 diff hist −1 Crystal growth: Tips and Tricks No edit summary
- 20:2320:23, 17 March 2008 diff hist −2 Crystal growth: Tips and Tricks No edit summary
- 20:1520:15, 17 March 2008 diff hist −2 Course or Conference 8 No edit summary
- 20:1520:15, 17 March 2008 diff hist +748 N Course or Conference 8 New page: This course will focus both on HTP crystallization, but the last day has a twist towards introducing [http://www.oppf.ox.ac.uk/xtalpims/ xtalPiMS], a spin-off of [http://www/pims-lims.org ...
- 20:0720:07, 17 March 2008 diff hist +82 Current events No edit summary
13 March 2008
- 16:1016:10, 13 March 2008 diff hist −73 Crystals Biophysical methods/// I tink when a page is made it should go to proteins
- 16:0616:06, 13 March 2008 diff hist +200 N User talk:Xtal1234 New page: Hi 1234 - Please look back to my changes for some tips in Wiki formating. Use == and === to make stad headers and tables of contents will appear. --~~~~ current
- 16:0416:04, 13 March 2008 diff hist −90 m Modifying the protein to crystallize better Use Wiki === headers, not 1. 2. ... and auto-make table of contents ...
- 16:0116:01, 13 March 2008 diff hist −14 Modifying the protein to crystallize better Editorial changes - please dont use xxx unless you want to create a page with that name!
7 March 2008
- 00:1100:11, 7 March 2008 diff hist +7 Crystallography →By package
- 00:1000:10, 7 March 2008 diff hist +7 Substructure determination No edit summary current
- 00:0900:09, 7 March 2008 diff hist +6 Crystallography →For Specific Tasks
- 00:0600:06, 7 March 2008 diff hist +11 Crystallography →Automated Pipelines for Structure Solution
- 00:0200:02, 7 March 2008 diff hist +429 Crystallography No edit summary
6 March 2008
- 23:5923:59, 6 March 2008 diff hist +38 N Model building (automated) Model building (automated) moved to Automated Model building: get rid of brackets in wiki link - not nice current
- 23:5923:59, 6 March 2008 diff hist 0 m Automated Model building Model building (automated) moved to Automated Model building: get rid of brackets in wiki link - not nice
- 23:5723:57, 6 March 2008 diff hist +34 N Experimental phasing (SIR/MIR and SAD/MAD) Experimental phasing (SIR/MIR and SAD/MAD) moved to Experimental phasing: The title was much too long with no reason to include the bracket in the title ... current
- 23:5723:57, 6 March 2008 diff hist 0 m Experimental phasing Experimental phasing (SIR/MIR and SAD/MAD) moved to Experimental phasing: The title was much too long with no reason to include the bracket in the title ...
18 February 2008
- 20:0320:03, 18 February 2008 diff hist 0 Main Page No edit summary
- 20:0320:03, 18 February 2008 diff hist 0 Main Page No edit summary
- 20:0320:03, 18 February 2008 diff hist +24 m Main Page No edit summary
- 20:0220:02, 18 February 2008 diff hist 0 m Main Page No edit summary
- 15:1815:18, 18 February 2008 diff hist −1 m Course or Conference 4 No edit summary
- 15:1815:18, 18 February 2008 diff hist +1,227 N Course or Conference 4 New page: The goal of the course is to present methods of macromolecular crystallization and give hands-on experience with them. Participants may bring their own proteins. The course is divided in...
- 15:1615:16, 18 February 2008 diff hist +98 Current events new course
- 15:1315:13, 18 February 2008 diff hist +91 m Current events chronological order!
- 15:1115:11, 18 February 2008 diff hist +28 N Courses and Conferences Courses and Conferences moved to Current events: fits there a bit better really - consensus with Kay current
- 15:1115:11, 18 February 2008 diff hist 0 m Current events Courses and Conferences moved to Current events: fits there a bit better really - consensus with Kay
- 14:5814:58, 18 February 2008 diff hist +207 N User talk:Kay New page: Kay - should I maybe move the courses and conferences to the Current Events space ? I would also be inclined to display it with headers and no separate pages for each. Give me the green ...
- 14:5414:54, 18 February 2008 diff hist +615 N Course or Conference 3 New page: The current trend in structural biology is to study macromolecular assemblies of ever-increasing size and complexity. This EMBO practical course aims to teach the strategic use of biochemi...
- 14:5314:53, 18 February 2008 diff hist +109 Current events No edit summary
- 14:4314:43, 18 February 2008 diff hist −3 m Main Page No edit summary
- 14:4314:43, 18 February 2008 diff hist −17 m Main Page No edit summary
- 14:4114:41, 18 February 2008 diff hist +774 Course or Conference 2 No edit summary
- 14:3914:39, 18 February 2008 diff hist +118 Current events No edit summary
- 12:5212:52, 18 February 2008 diff hist 0 m Main Page Most recent first !
- 12:3512:35, 18 February 2008 diff hist +2 m Visualization: graphics cards and 3D →graphics cards and drivers
- 12:3512:35, 18 February 2008 diff hist +1,918 Visualization: graphics cards and 3D Added dual desktop support for Nvidia drivers - might be superseded with new driver versions!
- 12:2812:28, 18 February 2008 diff hist +35 m Visualization: graphics cards and 3D introduced ,code> tags to the "code"
15 February 2008
- 14:2114:21, 15 February 2008 diff hist 0 m Topics →Expression and Purification
- 14:2014:20, 15 February 2008 diff hist +45 m User talk:Tassos No edit summary current
- 14:1914:19, 15 February 2008 diff hist +2 m SLIC cloning No edit summary
- 14:1414:14, 15 February 2008 diff hist +120 N SLIC cloning New page: The Heidelberg protocol ... The Milan protocol ... The Paris protocol ... A contribution from 3D-Repertoire
- 14:1314:13, 15 February 2008 diff hist +19 m Topics →Expression and Purification
12 February 2008
- 18:3418:34, 12 February 2008 diff hist 0 Expression systems →Mammalian cells current
- 18:3318:33, 12 February 2008 diff hist +320 m Expression systems →Human Embryonic Kidney (HEK) 293 cultured cells
- 18:3018:30, 12 February 2008 diff hist +719 Expression systems →Human Embryonic Kidney (HEK) 293 cultured cells
- 18:2118:21, 12 February 2008 diff hist +224 N User:Tassos New page: '''Anastassis (Tassos) Perrakis ''' Principal Investigator / Staff Member Netherlands Cancer Institute, Dept. H2 1066 CX Amsterdam, The Netherlands Tel: +31 20 512 1951 Fax: +31 20 512... current
- 18:1918:19, 12 February 2008 diff hist +137 N User talk:Rogerdodd New page: - Hi Artem: why do you use this format that put sthings in this dot-line box ? Could you please explain ? i think I am missing something.
- 18:0318:03, 12 February 2008 diff hist +24 Expression systems Changed to a more of a chpater format to encourage people to post more details !?!?
- 16:4916:49, 12 February 2008 diff hist +334 Expression systems →Mammalian cells
- 16:4016:40, 12 February 2008 diff hist +16 m Coot Moved the coot to the side of the contents - again aesthetics ...?
- 16:3616:36, 12 February 2008 diff hist −9 m Main Page a detail for coot - maybe it reads better now
11 February 2008
- 19:1119:11, 11 February 2008 diff hist +207 N Servers and programs for sequence analysis added my favorite tools -biased
- 19:1019:10, 11 February 2008 diff hist +182 N Structure based sequence alignment a few servers to start with ...
- 19:0919:09, 11 February 2008 diff hist +24 m Topics →Bioinformatics
10 February 2008
- 13:5913:59, 10 February 2008 diff hist +292 N ARP/wARP New page: ARP/wARP is a software suite for automated model building in X-ray crystallography. The [http://www.arp-warp.org ARP/wARP main page] has download instructions, a FAQ, and the User Guide. A...
- 13:5113:51, 10 February 2008 diff hist +234 m Modifying the protein to crystallize better →[[Lysine Methylation]]
- 13:5013:50, 10 February 2008 diff hist +216 m Modifying the protein to crystallize better →[[Lysine Methylation]]
- 13:5013:50, 10 February 2008 diff hist −217 m Lysine Methylation No edit summary
- 13:4913:49, 10 February 2008 diff hist +3 m Modifying the protein to crystallize better No edit summary
- 13:4913:49, 10 February 2008 diff hist −2 m Crystallization screens and methods No edit summary
- 13:4813:48, 10 February 2008 diff hist +707 Expression and Purification: Tips and Tricks No edit summary
- 13:4513:45, 10 February 2008 diff hist +1,055 N Crystallization screens and methods New page: * The PACT screen The PACT screen was developed by Janet Newman in the lab of Tassos Perrakis. This systematic screen, a pH-, anion- and cation-testing (PACT) screen, aims to decouple ...
- 13:2613:26, 10 February 2008 diff hist +838 N Lysine Methylation New page: This protocol is an NKI local adaptation based on the article by Grimes and co-workers[http://www.ncbi.nlm.nih.gov/pubmed/17098187?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_Re...
- 13:2413:24, 10 February 2008 diff hist +24 N Modifying the protein to crystallize better New page: * Lysine Methylation
- 13:2313:23, 10 February 2008 diff hist +50 m Topics →Crystal growth and manipulation
- 13:1513:15, 10 February 2008 diff hist +312 m Main Page a lits of chapter under "topics" is now added in front page, linked to the chapters
- 12:5812:58, 10 February 2008 diff hist +2,639 N Spheroplasts Plates New page: ==Day 0: Overnight growth of 96 selected clones== *Starting from rearraying list, perform an overnight culture of 96 clones in 1 ml of LB with antibiotics to taste. *To avoid any mistakes... current
- 12:5712:57, 10 February 2008 diff hist +2,062 N Spheroplasts Falcon New page: ==Spheroplasts lysis starting from Falcon tube cultures== *Prepare 50 ml falcon tubes with 12.5ml of medium plus antibiotics. *Transfer 125 uL of a saturated preculture. *Put falcons in i... current
- 12:5712:57, 10 February 2008 diff hist +221 N Expression screening New page: ==Expression Testing== *Cell lysis with Spheroplasts in 96-well Plates - From Darren Hart - Grenoble *[[Spheroplasts Falcon|Cell lysis with Spheroplasts in Falcon ... current
- 12:5312:53, 10 February 2008 diff hist +27 Topics →Expression and Purification
- 12:5212:52, 10 February 2008 diff hist 0 Topics →by package
- 12:5212:52, 10 February 2008 diff hist +15 Topics →by package