User contributions
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Jump to navigationJump to search- 09:43, 23 February 2009 diff hist -4 m Experimental phasing
- 09:42, 23 February 2009 diff hist +435 Experimental phasing Add CNS (shame on me for forgetting) and minor changes
- 09:31, 12 February 2009 diff hist +3,144 Experimental phasing a first try
- 22:15, 29 January 2009 diff hist +168 N Mapping sequence alignment to a structure New page: [http://consurf.tau.ac.il/ ConSurf] web server [http://www.mcgnmr.ca/ProtSkin/intro/ ProtSkin] web server [http://www.ncbi.nlm.nih.gov/pubmed/11524371 AL2CO] software
- 22:10, 29 January 2009 diff hist +48 Bioinformatics
- 13:11, 2 September 2008 diff hist +7,076 N LIC cloning New page: '''LIC-cloning into the pET-NKI LIC vectors''' [contributed by Vangelis Christodoulou, NKI] This protocol is based on the "version 2" LIC system designed by Vangelis. '''DAY -20 to -10...
- 13:09, 2 September 2008 diff hist +18 m Expression and Purification
- 11:58, 19 June 2008 diff hist +1,924 N The Milan protocol ... New page: =SLIC Sub-cloning using T4 DNA polymerase treated insert= * Digest 3 μg vector with two restriction enzymes. Gel-purify the vector and isolate the DNA using Qiagen extraction kit. Quanti...
- 07:07, 18 June 2008 diff hist +109 Test set cosmetics and wiki links
- 07:05, 18 June 2008 diff hist +34 Test set →How precise is the estimate of Rfree for a certain number of test set reflections?
- 22:41, 28 March 2008 diff hist +67 Structure based sequence alignment
- 18:31, 22 March 2008 diff hist -37 m Tags changed appearance from code to tabbed list
- 18:29, 22 March 2008 diff hist +7 m Positions Same as for Events
- 18:28, 22 March 2008 diff hist +5 m Current events
- 18:27, 22 March 2008 diff hist 0 m Current events the instructions go to the end ?
- 08:14, 22 March 2008 diff hist +690 ARP/wARP current
- 18:24, 17 March 2008 diff hist -1 Crystal growth: Tips and Tricks
- 18:23, 17 March 2008 diff hist -2 Crystal growth: Tips and Tricks
- 18:15, 17 March 2008 diff hist -2 Course or Conference 8
- 18:15, 17 March 2008 diff hist +748 N Course or Conference 8 New page: This course will focus both on HTP crystallization, but the last day has a twist towards introducing [http://www.oppf.ox.ac.uk/xtalpims/ xtalPiMS], a spin-off of [http://www/pims-lims.org ...
- 18:07, 17 March 2008 diff hist +82 Current events
- 14:10, 13 March 2008 diff hist -73 Crystals Biophysical methods/// I tink when a page is made it should go to proteins
- 14:06, 13 March 2008 diff hist +200 N User talk:Xtal1234 New page: Hi 1234 - Please look back to my changes for some tips in Wiki formating. Use == and === to make stad headers and tables of contents will appear. --~~~~ current
- 14:04, 13 March 2008 diff hist -90 m Modifying the protein to crystallize better Use Wiki === headers, not 1. 2. ... and auto-make table of contents ...
- 14:01, 13 March 2008 diff hist -14 Modifying the protein to crystallize better Editorial changes - please dont use xxx unless you want to create a page with that name!
- 22:11, 6 March 2008 diff hist +7 Crystallography →By package
- 22:10, 6 March 2008 diff hist +7 Substructure determination current
- 22:09, 6 March 2008 diff hist +6 Crystallography →For Specific Tasks
- 22:06, 6 March 2008 diff hist +11 Crystallography →Automated Pipelines for Structure Solution
- 22:02, 6 March 2008 diff hist +429 Crystallography
- 21:59, 6 March 2008 diff hist +38 N Model building (automated) Model building (automated) moved to Automated Model building: get rid of brackets in wiki link - not nice current
- 21:59, 6 March 2008 diff hist 0 m Automated Model building Model building (automated) moved to Automated Model building: get rid of brackets in wiki link - not nice
- 21:57, 6 March 2008 diff hist +34 N Experimental phasing (SIR/MIR and SAD/MAD) Experimental phasing (SIR/MIR and SAD/MAD) moved to Experimental phasing: The title was much too long with no reason to include the bracket in the title ... current
- 21:57, 6 March 2008 diff hist 0 m Experimental phasing Experimental phasing (SIR/MIR and SAD/MAD) moved to Experimental phasing: The title was much too long with no reason to include the bracket in the title ...
- 18:03, 18 February 2008 diff hist 0 Main Page
- 18:03, 18 February 2008 diff hist 0 Main Page
- 18:03, 18 February 2008 diff hist +24 m Main Page
- 18:02, 18 February 2008 diff hist 0 m Main Page
- 13:18, 18 February 2008 diff hist -1 m Course or Conference 4
- 13:18, 18 February 2008 diff hist +1,227 N Course or Conference 4 New page: The goal of the course is to present methods of macromolecular crystallization and give hands-on experience with them. Participants may bring their own proteins. The course is divided in...
- 13:16, 18 February 2008 diff hist +98 Current events new course
- 13:13, 18 February 2008 diff hist +91 m Current events chronological order!
- 13:11, 18 February 2008 diff hist +28 N Courses and Conferences Courses and Conferences moved to Current events: fits there a bit better really - consensus with Kay current
- 13:11, 18 February 2008 diff hist 0 m Current events Courses and Conferences moved to Current events: fits there a bit better really - consensus with Kay
- 12:58, 18 February 2008 diff hist +207 N User talk:Kay New page: Kay - should I maybe move the courses and conferences to the Current Events space ? I would also be inclined to display it with headers and no separate pages for each. Give me the green ...
- 12:54, 18 February 2008 diff hist +615 N Course or Conference 3 New page: The current trend in structural biology is to study macromolecular assemblies of ever-increasing size and complexity. This EMBO practical course aims to teach the strategic use of biochemi...
- 12:53, 18 February 2008 diff hist +109 Current events
- 12:43, 18 February 2008 diff hist -3 m Main Page
- 12:43, 18 February 2008 diff hist -17 m Main Page
- 12:41, 18 February 2008 diff hist +774 Course or Conference 2
- 12:39, 18 February 2008 diff hist +118 Current events
- 10:52, 18 February 2008 diff hist 0 m Main Page Most recent first !
- 10:35, 18 February 2008 diff hist +2 m Visualization: graphics cards and 3D →graphics cards and drivers
- 10:35, 18 February 2008 diff hist +1,918 Visualization: graphics cards and 3D Added dual desktop support for Nvidia drivers - might be superseded with new driver versions!
- 10:28, 18 February 2008 diff hist +35 m Visualization: graphics cards and 3D introduced ,code> tags to the "code"
- 12:21, 15 February 2008 diff hist 0 m Topics →Expression and Purification
- 12:20, 15 February 2008 diff hist +45 m User talk:Tassos current
- 12:19, 15 February 2008 diff hist +2 m SLIC cloning
- 12:14, 15 February 2008 diff hist +120 N SLIC cloning New page: The Heidelberg protocol ... The Milan protocol ... The Paris protocol ... A contribution from 3D-Repertoire
- 12:13, 15 February 2008 diff hist +19 m Topics →Expression and Purification
- 16:34, 12 February 2008 diff hist 0 Expression systems →Mammalian cells current
- 16:33, 12 February 2008 diff hist +320 m Expression systems →Human Embryonic Kidney (HEK) 293 cultured cells
- 16:30, 12 February 2008 diff hist +719 Expression systems →Human Embryonic Kidney (HEK) 293 cultured cells
- 16:21, 12 February 2008 diff hist +224 N User:Tassos New page: '''Anastassis (Tassos) Perrakis ''' Principal Investigator / Staff Member Netherlands Cancer Institute, Dept. H2 1066 CX Amsterdam, The Netherlands Tel: +31 20 512 1951 Fax: +31 20 512... current
- 16:19, 12 February 2008 diff hist +137 N User talk:Rogerdodd New page: - Hi Artem: why do you use this format that put sthings in this dot-line box ? Could you please explain ? i think I am missing something.
- 16:03, 12 February 2008 diff hist +24 Expression systems Changed to a more of a chpater format to encourage people to post more details !?!?
- 14:49, 12 February 2008 diff hist +334 Expression systems →Mammalian cells
- 14:40, 12 February 2008 diff hist +16 m Coot Moved the coot to the side of the contents - again aesthetics ...?
- 14:36, 12 February 2008 diff hist -9 m Main Page a detail for coot - maybe it reads better now
- 17:11, 11 February 2008 diff hist +207 N Servers and programs for sequence analysis added my favorite tools -biased
- 17:10, 11 February 2008 diff hist +182 N Structure based sequence alignment a few servers to start with ...
- 17:09, 11 February 2008 diff hist +24 m Topics →Bioinformatics
- 11:59, 10 February 2008 diff hist +292 N ARP/wARP New page: ARP/wARP is a software suite for automated model building in X-ray crystallography. The [http://www.arp-warp.org ARP/wARP main page] has download instructions, a FAQ, and the User Guide. A...
- 11:51, 10 February 2008 diff hist +234 m Modifying the protein to crystallize better →[[Lysine Methylation]]
- 11:50, 10 February 2008 diff hist +216 m Modifying the protein to crystallize better →[[Lysine Methylation]]
- 11:50, 10 February 2008 diff hist -217 m Lysine Methylation
- 11:49, 10 February 2008 diff hist +3 m Modifying the protein to crystallize better
- 11:49, 10 February 2008 diff hist -2 m Crystallization screens and methods
- 11:48, 10 February 2008 diff hist +707 Expression and Purification: Tips and Tricks
- 11:45, 10 February 2008 diff hist +1,055 N Crystallization screens and methods New page: * The PACT screen The PACT screen was developed by Janet Newman in the lab of Tassos Perrakis. This systematic screen, a pH-, anion- and cation-testing (PACT) screen, aims to decouple ...
- 11:26, 10 February 2008 diff hist +838 N Lysine Methylation New page: This protocol is an NKI local adaptation based on the article by Grimes and co-workers[http://www.ncbi.nlm.nih.gov/pubmed/17098187?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_Re...
- 11:24, 10 February 2008 diff hist +24 N Modifying the protein to crystallize better New page: * Lysine Methylation
- 11:23, 10 February 2008 diff hist +50 m Topics →Crystal growth and manipulation
- 11:15, 10 February 2008 diff hist +312 m Main Page a lits of chapter under "topics" is now added in front page, linked to the chapters
- 10:58, 10 February 2008 diff hist +2,639 N Spheroplasts Plates New page: ==Day 0: Overnight growth of 96 selected clones== *Starting from rearraying list, perform an overnight culture of 96 clones in 1 ml of LB with antibiotics to taste. *To avoid any mistakes... current
- 10:57, 10 February 2008 diff hist +2,062 N Spheroplasts Falcon New page: ==Spheroplasts lysis starting from Falcon tube cultures== *Prepare 50 ml falcon tubes with 12.5ml of medium plus antibiotics. *Transfer 125 uL of a saturated preculture. *Put falcons in i... current
- 10:57, 10 February 2008 diff hist +221 N Expression screening New page: ==Expression Testing== *Cell lysis with Spheroplasts in 96-well Plates - From Darren Hart - Grenoble *[[Spheroplasts Falcon|Cell lysis with Spheroplasts in Falcon ... current
- 10:53, 10 February 2008 diff hist +27 Topics →Expression and Purification
- 10:52, 10 February 2008 diff hist 0 Topics →by package
- 10:52, 10 February 2008 diff hist +15 Topics →by package