PIPE cloning

The Polymerase Incomplete Primer Extension (PIPE) method for cloning and mutagenesis used extensively at the Joint Center for Structural Genomics.

Heath E. Klock, Eric J. Koesema, Mark W. Knuth and Scott A. Lesley. Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts. Proteins 71, 982-994 (2008). doi:10.1002/prot.21786

For the detailed protcol, see
Klock HE, Lesley SA.(2009) The Polymerase Incomplete Primer Extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis. Methods Mol Biol. 498:91-103. doi:10.1007/978-1-59745-196-3_6

For an overview of the process, see the protein structure initiative knowledgebase research advances article “Speedy cloning and mutagenesis” doi:10.1038/th_psisgkb.2008.24