https://wiki.uni-konstanz.de/ccp4/index.php?title=PIPE_cloning&feed=atom&action=historyPIPE cloning - Revision history2024-03-29T06:15:08ZRevision history for this page on the wikiMediaWiki 1.39.6https://wiki.uni-konstanz.de/ccp4/index.php?title=PIPE_cloning&diff=1492&oldid=prevMmiller: New page: The Polymerase Incomplete Primer Extension (PIPE) method for cloning and mutagenesis used extensively at the [http://www.jcsg.org Joint Center for Structural Genomics]. Heath E. Klock, ...2009-07-25T21:46:21Z<p>New page: The Polymerase Incomplete Primer Extension (PIPE) method for cloning and mutagenesis used extensively at the [http://www.jcsg.org Joint Center for Structural Genomics]. Heath E. Klock, ...</p>
<p><b>New page</b></p><div>The Polymerase Incomplete Primer Extension (PIPE) method for cloning and mutagenesis<br />
used extensively at the [http://www.jcsg.org Joint Center for Structural Genomics]. <br />
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Heath E. Klock, Eric J. Koesema, Mark W. Knuth and Scott A. Lesley. Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts. <br />
''Proteins'' '''71''', 982-994 (2008). [http://dx.doi.org/10.1002/prot.21786 doi:10.1002/prot.21786]<br />
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For the detailed protcol, see <BR><br />
Klock HE, Lesley SA.(2009) The Polymerase Incomplete Primer Extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis. ''Methods Mol Biol.'' '''498''':91-103. [http://dx.doi.org/10.1007/978-1-59745-196-3_6 doi:10.1007/978-1-59745-196-3_6]<br />
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For an overview of the process, see the protein structure initiative knowledgebase research advances article<br />
“Speedy cloning and mutagenesis” [http://dx.doi.org/10.1038/th_psisgkb.2008.24 doi:10.1038/th_psisgkb.2008.24]</div>Mmiller