Difference between revisions of "Modifying the protein to crystallize better"

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== [[Lysine Methylation]]==
 
== [[Lysine Methylation]]==
This protocol is an NKI local adaptation based on the article by Grimes and co-workers[http://www.ncbi.nlm.nih.gov/pubmed/17098187?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum]
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This protocol is an NKI local adaptation based on the article by Grimes and co-workers[http://www.ncbi.nlm.nih.gov/pubmed/17098187?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum]. The idea is to change the charge properties post-purification, modify the solubility of the protein (hence the phase diagram) and get crystals! Its a quick and easy thing to try especially for proteins that appear to be too soluble!

Revision as of 11:51, 10 February 2008

Lysine Methylation

This protocol is an NKI local adaptation based on the article by Grimes and co-workers[1]. The idea is to change the charge properties post-purification, modify the solubility of the protein (hence the phase diagram) and get crystals! Its a quick and easy thing to try especially for proteins that appear to be too soluble!