Expression systems

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Revision as of 08:35, 11 February 2008 by Kay (talk | contribs)
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Bacteria

  Escherichia coli
  Bacillus subtilis
  Bacillus megaterium
  Pseudomonas fluorescens

Protozoa

  Leishmania tarentolae

Fungi

  Pichia pastoris
  Saccharomyces cerevisiae
  Schizosaccharomyces pombe
  Hansenula polymorpha
  Filamentous fungi

Insect cells

  Baculovirus-mediated expression in "Spodoptera frugiperda" and "Trichoplusia ni" cells

Mammalian cells

  Chinese hamster ovary cultured cells

Plant cells

  Algae
  Chloroplasts
Nicotiana tabacum

Try Rosetta cells

If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well. Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem. For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest".


--Artem 02:43, 11 February 2008 (CET)