Difference between revisions of "Expression systems"

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(Changed to a more of a chpater format to encourage people to post more details !?!?)
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=Bacteria=
+
=Mainstream systems=
  
  ''Escherichia coli''
+
==''Escherichia coli''==
  ''Bacillus subtilis''
+
===Rosetta cells ===
  ''Bacillus megaterium''
 
  ''Pseudomonas fluorescens''
 
  
=Protozoa=
+
If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well.  Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem.  For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest".
  ''Leishmania tarentolae''
+
 
 +
==Fungi==
 +
 
 +
===''Pichia pastoris''===
 +
 
 +
 
 +
==Insect cells==
 +
 
 +
===Baculovirus-mediated expression in ''Spodoptera frugiperd'' and ''Trichoplusia ni'' cells===
 +
 
 +
==Mammalian cells==
 +
 
 +
===Chinese hamster ovary (CHO) cultured cells===
 +
 
 +
===Human Embryonic Kidney (HEK) 293 cultured cells ===
 +
*[http://www.ncbi.nlm.nih.gov/pubmed/17001101?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Transient transfection].
 +
 
 +
*[http://www.invitrogen.com/content.cfm?pageid=3375 Flp-In for stable cell lines ].
 +
 
 +
=Other systems=
 +
 
 +
==Bacteria==
 +
 
 +
===''Bacillus subtilis''===
 +
 
 +
===''Bacillus megaterium''===
 +
 
 +
===''Pseudomonas fluorescens''===
 +
 
 +
==Protozoa==
 +
 
 +
===''Leishmania tarentolae''===
  
=Fungi=
+
==Fungi==
  ''Pichia pastoris''
 
  ''Saccharomyces cerevisiae''
 
  ''Schizosaccharomyces pombe''
 
  ''Hansenula polymorpha''
 
  Filamentous fungi
 
  
=Insect cells=
+
===''Saccharomyces cerevisiae''===
  Baculovirus-mediated expression in "Spodoptera frugiperda" and "Trichoplusia ni" cells
+
 
 +
===''Schizosaccharomyces pombe''===
 +
 
 +
===''Hansenula polymorpha''===
 +
 
 +
===''Filamentous fungi''===
  
=Mammalian cells=
 
  
  Chinese hamster ovary (CHO) cultured cells
 
  Human Embryonic Kidney (HEK) 293 cultured cells [http://www.ncbi.nlm.nih.gov/pubmed/17001101?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum (Transient transfection)].
 
  Human Embryonic Kidney (HEK) 293 cultured cells [http://www.invitrogen.com/content.cfm?pageid=3375 (Flp-In System)].
 
  
 
=Plant cells=
 
=Plant cells=
  Algae
 
  Chloroplasts
 
''Nicotiana tabacum''
 
  
= Try Rosetta cells =
+
==Algae==
  
If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well.  Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem.  For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest".
+
Chloroplasts
  
 
+
''Nicotiana tabacum''
--[[User:Zelig ff|Artem]] 02:43, 11 February 2008 (CET)
 

Revision as of 16:03, 12 February 2008

Mainstream systems

Escherichia coli

Rosetta cells

If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well. Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem. For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest".

Fungi

Pichia pastoris

Insect cells

Baculovirus-mediated expression in Spodoptera frugiperd and Trichoplusia ni cells

Mammalian cells

Chinese hamster ovary (CHO) cultured cells

Human Embryonic Kidney (HEK) 293 cultured cells

Other systems

Bacteria

Bacillus subtilis

Bacillus megaterium

Pseudomonas fluorescens

Protozoa

Leishmania tarentolae

Fungi

Saccharomyces cerevisiae

Schizosaccharomyces pombe

Hansenula polymorpha

Filamentous fungi

Plant cells

Algae

Chloroplasts

Nicotiana tabacum