Expression systems: Difference between revisions
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Algae | Algae | ||
Chloroplasts | Chloroplasts | ||
== Try Rosetta cells == | |||
If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well. Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem. For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest". | |||
''Nicotiana tabacum'' | ''Nicotiana tabacum'' | ||
--[[User:Zelig ff|Artem]] 02:43, 11 February 2008 (CET) | --[[User:Zelig ff|Artem]] 02:43, 11 February 2008 (CET) | ||
Revision as of 10:33, 11 February 2008
Bacteria
Escherichia coli Bacillus subtilis Bacillus megaterium Pseudomonas fluorescens
Protozoa
Leishmania tarentolae
Fungi
Pichia pastoris Saccharomyces cerevisiae Schizosaccharomyces pombe Hansenula polymorpha Filamentous fungi
Insect cells
Baculovirus-mediated expression in "Spodoptera frugiperda" and "Trichoplusia ni" cells
Mammalian cells
Chinese hamster ovary cultured cells
Plant cells
Algae Chloroplasts
Try Rosetta cells
If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well. Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem. For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest".
Nicotiana tabacum
--Artem 02:43, 11 February 2008 (CET)