Data collection: Tips and Tricks

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Revision as of 09:14, 15 February 2009 by Kay (talk | contribs) (New page: == cryo == * PEG3350 seems to be little too large for freezing, particularly in presence of high salt concentration. However, PEG2000 is good enough as a cryo protectant. * Try oils ...)
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cryo

  • PEG3350 seems to be little too large for freezing, particularly in presence of high salt concentration. However, PEG2000 is good enough as a cryo protectant.
  • Try oils
  • Try stepwise increase from low concentrations of cryoprotectants to the required concentration, over several minutes/hours/days.
  • The larger the crystal, the longer should be the incubation in each step.
  • Small crystals often do better in terms of mosaicity increase than big crystals.