Difference between revisions of "Crystals"

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== Crystal Characterization ==
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* [[Birefringence]]
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* [[solvent content]]
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== Crystal Imperfections ==
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* [[Diffuse Scattering]]
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* [[Incommensurate Lattices]]
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* [[Examples of diffraction patterns showing particular features]]
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== Crystal Growth ==
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* [[Modifying the protein to crystallize better]]
 
* [[Modifying the protein to crystallize better]]
 
* [[Crystallization screens and methods]]
 
* [[Crystallization screens and methods]]
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* [[I have crystals, but are they salt?]]
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* [[Conditions prone to salt crystallization]]
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* [[Biophysical methods]] for evaluation of crystallization tendency
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* [[Improving crystal quality]]
 
* [[Robots for crystallization setup]]
 
* [[Robots for crystallization setup]]
* [[Biophysical methods]] for characterizing proteins and their crystals
 
 
* [[Visualization of crystal growth]]
 
* [[Visualization of crystal growth]]
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* [[Crystal mounting]]
 
* [[Robots for crystal mounting]]
 
* [[Robots for crystal mounting]]
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* [[Robots for crystallization monitoring]]
 
* [[Crystal growth: Tips and Tricks]]
 
* [[Crystal growth: Tips and Tricks]]
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* [[Crystal growth: Protein-DNA complexes]]
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* [[BMCD|Biomolecular crystallization database]]
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* Prediction of protein crystallizability: [http://ffas.burnham.org/XtalPred-cgi/xtal.pl XtalPred]
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* crystallization of soluble proteins (see also [[Improving crystal quality]])
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* crystallization of membrane proteins (see also [[Improving crystal quality#Improving diffraction quality of membrane protein crystals]])
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* relative humidity calculations of salt/PEG solutions - can also be used to calculate equivalent/matching osmotic pressure: http://go.esrf.eu/RH
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== Crystal handling ==
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* [[Cryo]]
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* [[Sticky crystals]]
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* [[Soaking with heavy atoms]]
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* [[Microseeding]]
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== Data collection ==
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* [[X-ray generators]]
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* [[Detectors]]
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* [[Synchrotrons]]
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* [[Strategies]]
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* [[Data quality]]
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* [[Radiation damage]]
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* [[some ways to calculate the radiation dose that a crystal has absorbed]]
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* [[Comparison of home source and synchrotron properties]]
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* [[Data collection: Tips and Tricks]]
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* [[Storage of diffraction data]]
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== References ==
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# Wiener MC (2004) A pedestrian guide to membrane protein crystallization. Methods 34(3), 364-372.
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# Loll PJ (2003) Membrane protein structural biology: the high throughput challenge. J. Struct. Biol. 142(1), 144-153.
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# Methods and Results in Crystallization of Membrane Proteins Iwata S (Ed.), San Diego, CA: International University Line. (2003)
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# Macromolecular Crystallography Protocols, Volume 1: Preparation and Crystallization of Macromolecules. Doublie S (Ed.), Totowa, NJ: Humana Press. (2007)
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# Structural Biology of Membrane Proteins. Grisshammer R, & Buchanan SK (Eds.), Cambridge, UK: Royal Society of Chemistry. (2006)
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# Macromolecular Crystallisation and Crystal Perfection. N E Chayen, J R Helliwell and E H Snell OUP 2010 [http://www.oxfordscholarship.com/oso/public/content/physics/9780199213252/toc.html]

Latest revision as of 06:56, 19 June 2020

Crystal Characterization[edit | edit source]

Crystal Imperfections[edit | edit source]

Crystal Growth[edit | edit source]

Crystal handling[edit | edit source]

Data collection[edit | edit source]

References[edit | edit source]

  1. Wiener MC (2004) A pedestrian guide to membrane protein crystallization. Methods 34(3), 364-372.
  2. Loll PJ (2003) Membrane protein structural biology: the high throughput challenge. J. Struct. Biol. 142(1), 144-153.
  3. Methods and Results in Crystallization of Membrane Proteins Iwata S (Ed.), San Diego, CA: International University Line. (2003)
  4. Macromolecular Crystallography Protocols, Volume 1: Preparation and Crystallization of Macromolecules. Doublie S (Ed.), Totowa, NJ: Humana Press. (2007)
  5. Structural Biology of Membrane Proteins. Grisshammer R, & Buchanan SK (Eds.), Cambridge, UK: Royal Society of Chemistry. (2006)
  6. Macromolecular Crystallisation and Crystal Perfection. N E Chayen, J R Helliwell and E H Snell OUP 2010 [1]