Crystallization screens and methods

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The PACT screen

The PACT screen was developed by Janet Newman in the lab of Tassos Perrakis. This systematic screen, a pH-, anion- and cation-testing (PACT) screen, aims to decouple the components of each condition and to provide information about the protein, even in the absence of crystals, rather than cover a wide crystallization space[1]. The PACT screen is available from Molecular Dimensions[2] and Qiagen [3] under license, and by Jena Biosciences[4].

The exact formulations and all information for how to make the PACT screen are available as supplementary material of the original article by Newman et al[5] and are available under Open Access policy.