Course or Conference 2

[Biophysical Characterization of Macromolecular Complexes, NKI Amsterdam, May 21-23, 2008] Structural Biology of macromolecular complexes needs to always be complemented by biophysical studies to understand the details of the molecular interactions that determine function. In this practical “hands-on” course we will examine in detail:

• Surface Plasmon Resonance (SPR). Determination of kinetic parameters of interaction (kon, koff) and affinity (KD) from time-resolution experiments; screening of ligand arrays for protein binding.
• Isothermal Titration Calorimetry (ITC). Determination of thermodynamic parameters of interactions (ΔG,ΔH) and (KD) from ligand titration experiments.
• Multi-Angle Laser Light Scattering (MALLS)Determination of molecular weight of complexes concurrent with size exclusion chromatography.

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