Difference between revisions of "Course or Conference 2"

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Structural Biology of macromolecular complexes needs to always be complemented
 +
by biophysical studies to understand the details of the molecular interactions
 +
that determine function. In this practical “hands-on” course we will
 +
examine in detail:
 +
*Surface Plasmon Resonance (SPR). Determination of kinetic parameters of interaction (kon, koff) and affinity (KD) from time-resolution experiments; screening of ligand arrays for protein binding.
 +
*Isothermal Titration Calorimetry (ITC). Determination of thermodynamic parameters of interactions (ΔG,ΔH) and (KD) from ligand titration experiments.
 +
*Multi-Angle Laser Light Scattering (MALLS)Determination of molecular weight of complexes concurrent with size exclusion chromatography.
 +
 
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[[http://xtal.nki.nl/BioCourse_08.pdf PDF poster]]

Revision as of 12:41, 18 February 2008

Structural Biology of macromolecular complexes needs to always be complemented by biophysical studies to understand the details of the molecular interactions that determine function. In this practical “hands-on” course we will examine in detail:

  • Surface Plasmon Resonance (SPR). Determination of kinetic parameters of interaction (kon, koff) and affinity (KD) from time-resolution experiments; screening of ligand arrays for protein binding.
  • Isothermal Titration Calorimetry (ITC). Determination of thermodynamic parameters of interactions (ΔG,ΔH) and (KD) from ligand titration experiments.
  • Multi-Angle Laser Light Scattering (MALLS)Determination of molecular weight of complexes concurrent with size exclusion chromatography.

[PDF poster]