Difference between revisions of "Course or Conference 2"

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[Biophysical Characterization of Macromolecular Complexes, NKI Amsterdam, May 21-23, 2008]
 
Structural Biology of macromolecular complexes needs to always be complemented
 
Structural Biology of macromolecular complexes needs to always be complemented
 
by biophysical studies to understand the details of the molecular interactions
 
by biophysical studies to understand the details of the molecular interactions

Revision as of 11:24, 19 February 2008

[Biophysical Characterization of Macromolecular Complexes, NKI Amsterdam, May 21-23, 2008] Structural Biology of macromolecular complexes needs to always be complemented by biophysical studies to understand the details of the molecular interactions that determine function. In this practical “hands-on” course we will examine in detail:

  • Surface Plasmon Resonance (SPR). Determination of kinetic parameters of interaction (kon, koff) and affinity (KD) from time-resolution experiments; screening of ligand arrays for protein binding.
  • Isothermal Titration Calorimetry (ITC). Determination of thermodynamic parameters of interactions (ΔG,ΔH) and (KD) from ligand titration experiments.
  • Multi-Angle Laser Light Scattering (MALLS)Determination of molecular weight of complexes concurrent with size exclusion chromatography.

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