Conditions prone to salt crystallization
For some background information please see I have crystals, but are they salt?.
This list shall not include instances when significant drying of the drop occurred. This list should not be taken to mean that the given conditions always produce salt crystals, but they apparently sometimes do.
|Screen and #||Condition||Protein buffer||Confirmed by diffraction||Signature and comment|
|Hampton PEG/ion #1||0.2M NaF, 20% PEG3350, pH7.1||10mM Tris pH7.5, 150mM NaCl||yes||I've seen this condition produce salt crystals at least twice.--Ed 16:18, 23 May 2008 (CEST)
Ditto - NaF has caused me trouble many times - buyer beware! DaveB 11:01, 24 May 2008 (CEST)
Plate 3MembFac & Natrix equivalent
|1M Ammonium Sulfate, 0.1M Sodium Acetate||25mM NaPO4 pH8, 150mM NaCl||yes||Crystals reproduced with proteins and with buffer alone at least twice --Emeric.gueneau 18:21, 16 July 2008 (CEST)|
|Qiagen/Nextal Anions #5 and #6||0.3 M and 0.6 M NaF in NaOAc pH 4.6||20mM Hepes pH7.5, 150mM NaCl||yes||Gives hexagon shaped crystals every time- see line one - beware with NaF --Tom Brett 15:35, 23 July 2008 (CEST)|
|Hampton Crystal Screen #38||0.1 M Hepes pH 7.5, 1.4 M Sodium Citrate||no||Sodium Citrate crystallizes at cold room temp|
|JCSG+ #1 (A1)||0.2M Li2SO4, 0.1M NaOAc pH 4.5, 50.0% v/v PEG-400||No||Mixture must be made by adding water first, else precipitates. Also, crystallizes/precipitates with 10mM CaCl in protein buffer, perhaps even less. Others report needle crystals in well solution.|
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