Conditions prone to salt crystallization: Difference between revisions

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<td>yes</td>
<td>yes</td>
<td>I've seen this condition produce salt crystals at least twice.--[[User:Pozharski|Ed]] 16:18, 23 May 2008 (CEST) <br> Ditto - NaF has caused me trouble many times - buyer beware! [[User:DaveB|DaveB]] 11:01, 24 May 2008 (CEST) </td>
<td>I've seen this condition produce salt crystals at least twice.--[[User:Pozharski|Ed]] 16:18, 23 May 2008 (CEST) <br> Ditto - NaF has caused me trouble many times - buyer beware! [[User:DaveB|DaveB]] 11:01, 24 May 2008 (CEST) </td>
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<td> Qiagen
Plate 3
MembFac & Natrix equivalent</td>
<td>1M Ammonium Sulfate, 0.1M Sodium Acetate </td>
<td>25mM NaPO4 pH8, 150mM NaCl </td>
<td>yes </td>
<td>Crystals reproduced with proteins and with buffer alone at least twice  </td>
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Revision as of 18:20, 16 July 2008

For some background information please see I have crystals, but are they salt?.

This list shall not include instances when significant drying of the drop occurred. This list should not be taken to mean that the given conditions always produce salt crystals, but they apparently sometimes do.

Screen and # Condition Protein buffer Confirmed by diffraction Signature and comment
Hampton PEG/ion #1 0.2M NaF, 20% PEG3350, pH7.1 10mM Tris pH7.5, 150mM NaCl yes I've seen this condition produce salt crystals at least twice.--Ed 16:18, 23 May 2008 (CEST)
Ditto - NaF has caused me trouble many times - buyer beware! DaveB 11:01, 24 May 2008 (CEST)
Qiagen

Plate 3

MembFac & Natrix equivalent
1M Ammonium Sulfate, 0.1M Sodium Acetate 25mM NaPO4 pH8, 150mM NaCl yes Crystals reproduced with proteins and with buffer alone at least twice


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