Difference between revisions of "Conditions prone to salt crystallization"

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<td>Qiagen/Nextal Anions #5 and #6</td>
 
<td>Qiagen/Nextal Anions #5 and #6</td>
 
<td>0.3 M and 0.6 M NaF in NaOAc pH 4.6 </td>
 
<td>0.3 M and 0.6 M NaF in NaOAc pH 4.6 </td>
<td>10mM Tris pH7.5, 150mM NaCl </td>
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<td>20mM Hepes pH7.5, 150mM NaCl </td>
 
<td>yes </td>
 
<td>yes </td>
<td>Gives hexagon shaped crystals every time- see line one - beware with NaF </td>
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<td>Gives hexagon shaped crystals every time- see line one - beware with NaF --[[User:Tom Brett|Tom Brett]] 15:35, 23 July 2008 (CEST)</td>
 
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Revision as of 13:35, 23 July 2008

For some background information please see I have crystals, but are they salt?.

This list shall not include instances when significant drying of the drop occurred. This list should not be taken to mean that the given conditions always produce salt crystals, but they apparently sometimes do.

Screen and # Condition Protein buffer Confirmed by diffraction Signature and comment
Hampton PEG/ion #1 0.2M NaF, 20% PEG3350, pH7.1 10mM Tris pH7.5, 150mM NaCl yes I've seen this condition produce salt crystals at least twice.--Ed 16:18, 23 May 2008 (CEST)
Ditto - NaF has caused me trouble many times - buyer beware! DaveB 11:01, 24 May 2008 (CEST)
Qiagen

Plate 3

MembFac & Natrix equivalent
1M Ammonium Sulfate, 0.1M Sodium Acetate 25mM NaPO4 pH8, 150mM NaCl yes Crystals reproduced with proteins and with buffer alone at least twice --Emeric.gueneau 18:21, 16 July 2008 (CEST)
Qiagen/Nextal Anions #5 and #6 0.3 M and 0.6 M NaF in NaOAc pH 4.6 20mM Hepes pH7.5, 150mM NaCl yes Gives hexagon shaped crystals every time- see line one - beware with NaF --Tom Brett 15:35, 23 July 2008 (CEST)


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