https://wiki.uni-konstanz.de/ccp4/api.php?action=feedcontributions&user=Jbosch&feedformat=atomCCP4 wiki - User contributions [en]2024-03-29T08:51:47ZUser contributionsMediaWiki 1.39.6https://wiki.uni-konstanz.de/ccp4/index.php?title=Synchrotrons&diff=1032Synchrotrons2008-07-24T06:36:05Z<p>Jbosch: </p>
<hr />
<div>For a list of synchrotrons please see [http://www.esrf.eu/UsersAndScience/Links/Synchrotrons Synchrotron beamlines]<br />
<br />
The following beamlines offer mail-in service or remote access:<br />
(compiled from the CCP4 BB July 2008)<br />
<br />
1. The ALS BCSB beamlines http://bcsb.lbl.gov/beamlines.htm have a mail-in type program name collaborative crystallography on beamlines 5.0.2 (MAD) and 5.0.1 (Se-SAD/ Fixed<br />
Wavelength).<br />
Remote access is currently available on the sector 5 stations (5.0.1<br />
5.0.2 and 5.0.3) for PRT members only. ALS 4.2.2 beamline has remote capability<br />
<br />
2. Beamline 3BM1 at the Australian Synchrotron http://www.synchrotron.org.au has full remote access available for users. We use the same Blu-Ice gui as SSRL and use the SAM robot.<br />
When 3ID is available for general user program (2009) it will also have full remote access capabilities.<br />
<br />
3. BNL / NSLS Mail-in program http://www.px.nsls.bnl.gov/, where three scientists spend about one full-time equivalent of effort, employing about one full-time beamline among the six PXRR beamlines. <br />
Access to Beam Time http://www.px.nsls.bnl.gov/beamtime_requests.html Mail-in Program http://www.px.nsls.bnl.gov/Mailin.html<br />
<br />
4. The canadian light source http://www.lightsource.ca/ has at least mail in protein crystallography. <br />
<br />
5. MacCHESS http://www.macchess.cornell.edu/ does "mail-in" collection on all PX beamlines.<br />
<br />
6. All the ESRF MX/PX beamlines (ID14-1, ID14-2, ID14-4, ID23-1, ID29), and the CRG beam-line BM14, have remote access.<br />
http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access<br />
For industrial users there is also the MXpress mail in service:<br />
http://www.esrf.eu/Industry/our_services/xpress-service/xpress-service<br />
<br />
7. SEC-CAT 22-ID and 22-BM at APS http://www.ser-cat.org/ will do both mail-in and remote<br />
<br />
8. SPring8 in Japan http://www.spring8.or.jp/en/about_us/organization/research_utilization/biology has a mail-in system.<br />
<br />
9. SSRL http://smb.slac.stanford.edu/ all PX beamlines have [http://smb.slac.stanford.edu/public/facilities/remote_access/ remote capability]. About 75% of the users use fully remote data collection routinely<br />
<br />
10. CAMD's PX beamline http://www.camd.lsu.edu/gcpcc/GCPCChome.htm has a mail-in program for both consortium members and outside users.<br />
<br />
11. BESSY BL14.1 and BL14.2 http://www.mx.bessy.de, The BESSY-MX group is offering the "mail-in" service for industrial collaborators.<br />
<br />
12. SLS (http://sls.web.psi.ch/): For industrial users there is a Mail-In Service available since more than 3 years. For technical questions please contact Joachim Diez (Joachim.Diez@psi.ch) or Clemens Schulze-Briese (clemens.schulze@psi.ch), for questions about contracts please contact Philipp Dietrich (philipp.dietrich@psi.ch). Remote operation is in preparation for all beamlines. <br />
<br />
A useful link from PDB containing synchrotron info:<br />
http://biosync.rcsb.org/<br />
<br />
<br />
<table border = "1"><br />
<tr><br />
<b><br />
<td>Synchrotron</td><br />
<td>Beamline</td><br />
<td>Mail-in/ Remote</td><br />
<td>type of puck</td><br />
<td>Fixed Wavelength/ Se-SAD/ MAD</td><br />
<td>URL</td><br />
</b></tr><br />
<tr><br />
<td>ALS</td><br />
<td>4.2.2 </td><br />
<td>Remote capabilities </td><br />
<td>ALS puck ? </td><br />
<td> ? </td><br />
<td>http://bcsb.lbl.gov/beamlines.htm </td><br />
</tr><br />
<tr><br />
<b><br />
<td>ALS</td><br />
<td>5.0.1</td><br />
<td>Mail & Remote (PRT members)</td><br />
<td>ALS puck</td><br />
<td>Fixed Wavelength/ Se-SAD/</td><br />
<td>http://bcsb.lbl.gov/beamlines.htm</td><br />
</b></tr><br />
<tr><br />
<td>ALS </td><br />
<td>5.0.2 </td><br />
<td>Mail & Remote (PRT members) </td><br />
<td>ALS puck </td><br />
<td>MAD </td><br />
<td>http://bcsb.lbl.gov/beamlines.htm </td><br />
</tr><br />
<tr><br />
<td>ALS</td><br />
<td>5.0.3 </td><br />
<td>Mail & Remote (PRT members) </td><br />
<td>ALS puck </td><br />
<td> ? </td><br />
<td>http://bcsb.lbl.gov/beamlines.htm </td><br />
</tr><br />
<br />
<tr><br />
<td>Australian Synchrotron </td><br />
<td>3BM1 </td><br />
<td>remote </td><br />
<td>SSRL cassettes ? </td><br />
<td>MAD ?</td><br />
<td>http://www.synchrotron.org.au/ </td><br />
</tr><br />
<br />
<tr><br />
<td>Australian Synchrotron </td><br />
<td>3ID (2009) </td><br />
<td>remote </td><br />
<td>SSRL cassettes ? </td><br />
<td>MAD ?</td><br />
<td>http://www.synchrotron.org.au/ </td><br />
</tr><br />
<br />
<tr><br />
<td>BNL/NSLS </td><br />
<td>six PXRR beamlines</td><br />
<td>Mail-in </td><br />
<td> ? </td><br />
<td> ? </td><br />
<td>http://www.px.nsls.bnl.gov/ http://www.px.nsls.bnl.gov/Mailin.html </td><br />
</tr><br />
<br />
<tr><br />
<td>CLS </td><br />
<td> ?</td><br />
<td>Mail-in </td><br />
<td>? </td><br />
<td>? </td><br />
<td>http://www.lightsource.ca/ </td><br />
</tr><br />
<br />
<tr><br />
<td>MacCHESS </td><br />
<td>all PX beamlines </td><br />
<td>Mail-in </td><br />
<td>ALS type puck ? </td><br />
<td>? </td><br />
<td>http://www.macchess.cornell.edu/ </td><br />
</tr><br />
<br />
<tr><br />
<td>ESRF </td><br />
<td>ID14-1 </td><br />
<td>Remote </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access </td><br />
</tr><br />
<br />
<tr><br />
<td>ESRF </td><br />
<td>ID14-2 </td><br />
<td>Remote </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access </td><br />
</tr><br />
<tr><br />
<td>ESRF </td><br />
<td>ID14-4 </td><br />
<td>Remote </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access </td><br />
</tr><br />
<tr><br />
<td>ESRF </td><br />
<td>ID23-1 </td><br />
<td>Remote </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access </td><br />
</tr><br />
<tr><br />
<td>ESRF </td><br />
<td>ID29 </td><br />
<td>Remote </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access </td><br />
</tr><br />
<tr><br />
<td>ESRF </td><br />
<td>BM14 </td><br />
<td>Remote </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access </td><br />
</tr><br />
<br />
<tr><br />
<td>ESRF </td><br />
<td>MXpress </td><br />
<td>Mail-in </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.esrf.eu/Industry/our_services/xpress-service/xpress-service </td><br />
</tr><br />
<br />
<tr><br />
<td>APS </td><br />
<td>SEC-CAT 22-ID </td><br />
<td>Mail-in & Remote </td><br />
<td>? </td><br />
<td>? </td><br />
<td>http://www.ser-cat.org/ </td><br />
</tr><br />
<br />
<tr><br />
<td>APS </td><br />
<td>SEC-CAT 22-BM </td><br />
<td>Mail-in & Remote </td><br />
<td>? </td><br />
<td>? </td><br />
<td>http://www.ser-cat.org/ </td><br />
</tr><br />
<br />
<tr><br />
<td>Spring8 </td><br />
<td>? </td><br />
<td>Mail-in </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.spring8.or.jp/en/about_us/organization/research_utilization/biology </td><br />
</tr><br />
<br />
<tr><br />
<td>SSRL </td><br />
<td>all PX beamlines </td><br />
<td>remote </td><br />
<td> SSRL cassette </td><br />
<td> ? </td><br />
<td>http://smb.slac.stanford.edu/ </td><br />
</tr><br />
<br />
<tr><br />
<td>CAMD </td><br />
<td>PX beamline </td><br />
<td>Mail-in </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.camd.lsu.edu/gcpcc/GCPCChome.htm </td><br />
</tr><br />
<br />
<tr><br />
<td>BESSY </td><br />
<td>BL14.1 </td><br />
<td>Mail-in (industrial) </td><br />
<td> ? </td><br />
<td> ?</td><br />
<td>http://www.mx.bessy.de/ </td><br />
</tr><br />
<br />
<tr><br />
<td>BESSY </td><br />
<td>BL14.2 </td><br />
<td>Mail-in (industrial) </td><br />
<td> ? </td><br />
<td> ?</td><br />
<td>http://www.mx.bessy.de/ </td><br />
</tr><br />
<br />
<tr><br />
<td>SLS </td><br />
<td> ?</td><br />
<td>Mail-in (industrial) </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://sls.web.psi.ch/ </td><br />
</tr><br />
<br />
<br />
<tr><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
</tr><br />
<br />
</table><br />
<br />
'''''Please copy and paste the empty row for the next person to fill in'''''</div>Jboschhttps://wiki.uni-konstanz.de/ccp4/index.php?title=Synchrotrons&diff=1031Synchrotrons2008-07-24T06:29:34Z<p>Jbosch: </p>
<hr />
<div>For a list of synchrotrons please see [http://www.esrf.eu/UsersAndScience/Links/Synchrotrons Synchrotron beamlines]<br />
<br />
The following beamlines offer mail-in service or remote access:<br />
(compiled from the CCP4 BB July 2008)<br />
<br />
1. The ALS BCSB beamlines http://bcsb.lbl.gov/beamlines.htm have a mail-in type program name collaborative crystallography on beamlines 5.0.2 (MAD) and 5.0.1 (Se-SAD/ Fixed<br />
Wavelength).<br />
Remote access is currently available on the sector 5 stations (5.0.1<br />
5.0.2 and 5.0.3) for PRT members only. ALS 4.2.2 beamline has remote capability<br />
<br />
2. Beamline 3BM1 at the Australian Synchrotron http://www.synchrotron.org.au has full remote access available for users. We use the same Blu-Ice gui as SSRL and use the SAM robot.<br />
When 3ID is available for general user program (2009) it will also have full remote access capabilities.<br />
<br />
3. BNL / NSLS Mail-in program http://www.px.nsls.bnl.gov/, where three scientists spend about one full-time equivalent of effort, employing about one full-time beamline among the six PXRR beamlines. <br />
Access to Beam Time http://www.px.nsls.bnl.gov/beamtime_requests.html Mail-in Program http://www.px.nsls.bnl.gov/Mailin.html<br />
<br />
4. The canadian light source http://www.lightsource.ca/ has at least mail in protein crystallography. <br />
<br />
5. MacCHESS http://www.macchess.cornell.edu/ does "mail-in" collection on all PX beamlines.<br />
<br />
6. All the ESRF MX/PX beamlines (ID14-1, ID14-2, ID14-4, ID23-1, ID29), and the CRG beam-line BM14, have remote access.<br />
http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access<br />
For industrial users there is also the MXpress mail in service:<br />
http://www.esrf.eu/Industry/our_services/xpress-service/xpress-service<br />
<br />
7. SEC-CAT 22-ID and 22-BM at APS http://www.ser-cat.org/ will do both mail-in and remote<br />
<br />
8. SPring8 in Japan http://www.spring8.or.jp/en/about_us/organization/research_utilization/biology has a mail-in system.<br />
<br />
9. SSRL http://smb.slac.stanford.edu/ all PX beamlines have [http://smb.slac.stanford.edu/public/facilities/remote_access/ remote capability]. About 75% of the users use fully remote data collection routinely<br />
<br />
10. CAMD's PX beamline http://www.camd.lsu.edu/gcpcc/GCPCChome.htm has a mail-in program for both consortium members and outside users.<br />
<br />
11. BESSY BL14.1 and BL14.2 http://www.mx.bessy.de, The BESSY-MX group is offering the "mail-in" service for industrial collaborators.<br />
<br />
12. SLS (http://sls.web.psi.ch/): For industrial users there is a Mail-In Service available since more than 3 years. For technical questions please contact Joachim Diez (Joachim.Diez@psi.ch) or Clemens Schulze-Briese (clemens.schulze@psi.ch), for questions about contracts please contact Philipp Dietrich (philipp.dietrich@psi.ch). Remote operation is in preparation for all beamlines. <br />
<br />
A useful link from PDB containing synchrotron info:<br />
http://biosync.rcsb.org/<br />
<br />
<br />
<table border = "1"><br />
<tr><br />
<b><br />
<td>Synchrotron</td><br />
<td>Beamline</td><br />
<td>Mail-in/ Remote</td><br />
<td>type of puck</td><br />
<td>Fixed Wavelength/ Se-SAD/ MAD</td><br />
<td>URL</td><br />
</b></tr><br />
<tr><br />
<td>ALS</td><br />
<td>4.2.2 </td><br />
<td>Remote capabilities </td><br />
<td>ALS puck ? </td><br />
<td> ? </td><br />
<td>http://bcsb.lbl.gov/beamlines.htm </td><br />
</tr><br />
<tr><br />
<b><br />
<td>ALS</td><br />
<td>5.0.1</td><br />
<td>Mail & Remote (PRT members)</td><br />
<td>ALS puck</td><br />
<td>Fixed Wavelength/ Se-SAD/</td><br />
<td>http://bcsb.lbl.gov/beamlines.htm</td><br />
</b></tr><br />
<tr><br />
<td>ALS </td><br />
<td>5.0.2 </td><br />
<td>Mail & Remote (PRT members) </td><br />
<td>ALS puck </td><br />
<td>MAD </td><br />
<td>http://bcsb.lbl.gov/beamlines.htm </td><br />
</tr><br />
<tr><br />
<td>ALS</td><br />
<td>5.0.3 </td><br />
<td>Mail & Remote (PRT members) </td><br />
<td>ALS puck </td><br />
<td> ? </td><br />
<td>http://bcsb.lbl.gov/beamlines.htm </td><br />
</tr><br />
<br />
<tr><br />
<td>Australian Synchrotron </td><br />
<td>3BM1 </td><br />
<td>remote </td><br />
<td>SSRL cassettes ? </td><br />
<td>MAD ?</td><br />
<td>http://www.synchrotron.org.au/ </td><br />
</tr><br />
<br />
<tr><br />
<td>Australian Synchrotron </td><br />
<td>3ID (2009) </td><br />
<td>remote </td><br />
<td>SSRL cassettes ? </td><br />
<td>MAD ?</td><br />
<td>http://www.synchrotron.org.au/ </td><br />
</tr><br />
<br />
<tr><br />
<td>BNL/NSLS </td><br />
<td>six PXRR beamlines</td><br />
<td>Mail-in </td><br />
<td> ? </td><br />
<td> ? </td><br />
<td>http://www.px.nsls.bnl.gov/ http://www.px.nsls.bnl.gov/Mailin.html </td><br />
</tr><br />
<br />
<tr><br />
<td>CLS </td><br />
<td> ?</td><br />
<td>Mail-in </td><br />
<td>? </td><br />
<td>? </td><br />
<td>http://www.lightsource.ca/ </td><br />
</tr><br />
<br />
<tr><br />
<td>MacCHESS </td><br />
<td>all PX beamlines </td><br />
<td>Mail-in </td><br />
<td>ALS type puck ? </td><br />
<td>? </td><br />
<td>http://www.macchess.cornell.edu/ </td><br />
</tr><br />
<br />
<tr><br />
<td>ESRF </td><br />
<td>ID14-1 </td><br />
<td>Remote </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access </td><br />
</tr><br />
<br />
<tr><br />
<td>ESRF </td><br />
<td>ID14-2 </td><br />
<td>Remote </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access </td><br />
</tr><br />
<tr><br />
<td>ESRF </td><br />
<td>ID14-4 </td><br />
<td>Remote </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access </td><br />
</tr><br />
<tr><br />
<td>ESRF </td><br />
<td>ID23-1 </td><br />
<td>Remote </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access </td><br />
</tr><br />
<tr><br />
<td>ESRF </td><br />
<td>ID29 </td><br />
<td>Remote </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access </td><br />
</tr><br />
<tr><br />
<td>ESRF </td><br />
<td>BM14 </td><br />
<td>Remote </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access </td><br />
</tr><br />
<br />
<tr><br />
<td>ESRF </td><br />
<td>MXpress </td><br />
<td>Mail-in </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.esrf.eu/Industry/our_services/xpress-service/xpress-service </td><br />
</tr><br />
<br />
<tr><br />
<td>APS </td><br />
<td>SEC-CAT 22-ID </td><br />
<td>Mail-in & Remote </td><br />
<td>? </td><br />
<td>? </td><br />
<td>http://www.ser-cat.org/ </td><br />
</tr><br />
<br />
<tr><br />
<td>APS </td><br />
<td>SEC-CAT 22-BM </td><br />
<td>Mail-in & Remote </td><br />
<td>? </td><br />
<td>? </td><br />
<td>http://www.ser-cat.org/ </td><br />
</tr><br />
<br />
<tr><br />
<td>Spring8 </td><br />
<td>? </td><br />
<td>Mail-in </td><br />
<td> ?</td><br />
<td> ?</td><br />
<td>http://www.spring8.or.jp/en/about_us/organization/research_utilization/biology </td><br />
</tr><br />
<br />
<br />
</table><br />
<br />
'''''Please copy and paste the empty row for the next person to fill in'''''</div>Jboschhttps://wiki.uni-konstanz.de/ccp4/index.php?title=Synchrotrons&diff=1030Synchrotrons2008-07-24T06:10:20Z<p>Jbosch: </p>
<hr />
<div>For a list of synchrotrons please see [http://www.esrf.eu/UsersAndScience/Links/Synchrotrons Synchrotron beamlines]<br />
<br />
The following beamlines offer mail-in service or remote access:<br />
(compiled from the CCP4 BB July 2008)<br />
<br />
1. The ALS BCSB beamlines http://bcsb.lbl.gov/beamlines.htm have a mail-in type program name collaborative crystallography on beamlines 5.0.2 (MAD) and 5.0.1 (Se-SAD/ Fixed<br />
Wavelength).<br />
Remote access is currently available on the sector 5 stations (5.0.1<br />
5.0.2 and 5.0.3) for PRT members only. ALS 4.2.2 beamline has remote capability<br />
<br />
2. Beamline 3BM1 at the Australian Synchrotron http://www.synchrotron.org.au has full remote access available for users. We use the same Blu-Ice gui as SSRL and use the SAM robot.<br />
When 3ID is available for general user program (2009) it will also have full remote access capabilities.<br />
<br />
3. BNL / NSLS Mail-in program http://www.px.nsls.bnl.gov/, where three scientists spend about one full-time equivalent of effort, employing about one full-time beamline among the six PXRR beamlines. <br />
Access to Beam Time http://www.px.nsls.bnl.gov/beamtime_requests.html Mail-in Program http://www.px.nsls.bnl.gov/Mailin.html<br />
<br />
4. The canadian light source http://www.lightsource.ca/ has at least mail in protein crystallography. <br />
<br />
5. MacCHESS http://www.macchess.cornell.edu/ does "mail-in" collection on all PX beamlines.<br />
<br />
6. All the ESRF MX/PX beamlines (ID14-1, ID14-2, ID14-4, ID23-1, ID29), and the CRG beam-line BM14, have remote access.<br />
http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access<br />
For industrial users there is also the MXpress mail in service:<br />
http://www.esrf.eu/Industry/our_services/xpress-service/xpress-service<br />
<br />
7. SEC-CAT 22-ID and 22-BM at APS http://www.ser-cat.org/ will do both mail-in and remote<br />
<br />
8. SPring8 in Japan http://www.spring8.or.jp/en/about_us/organization/research_utilization/biology has a mail-in system.<br />
<br />
9. SSRL http://smb.slac.stanford.edu/ all PX beamlines have [http://smb.slac.stanford.edu/public/facilities/remote_access/ remote capability]. About 75% of the users use fully remote data collection routinely<br />
<br />
10. CAMD's PX beamline http://www.camd.lsu.edu/gcpcc/GCPCChome.htm has a mail-in program for both consortium members and outside users.<br />
<br />
11. BESSY BL14.1 and BL14.2 http://www.mx.bessy.de, The BESSY-MX group is offering the "mail-in" service for industrial collaborators.<br />
<br />
12. SLS (http://sls.web.psi.ch/): For industrial users there is a Mail-In Service available since more than 3 years. For technical questions please contact Joachim Diez (Joachim.Diez@psi.ch) or Clemens Schulze-Briese (clemens.schulze@psi.ch), for questions about contracts please contact Philipp Dietrich (philipp.dietrich@psi.ch). Remote operation is in preparation for all beamlines. <br />
<br />
A useful link from PDB containing synchrotron info:<br />
http://biosync.rcsb.org/<br />
<br />
<br />
<table border = "1"><br />
<tr><br />
<b><br />
<td>Synchrotron</td><br />
<td>Beamline</td><br />
<td>Mail-in/ Remote</td><br />
<td>type of puck</td><br />
<td>Fixed Wavelength/ Se-SAD/ MAD</td><br />
<td>URL</td><br />
</b></tr><br />
<tr><br />
<b><br />
<td>ALS</td><br />
<td>5.0.1</td><br />
<td>Mail & Remote (PRT members)</td><br />
<td>ALS puck</td><br />
<td>Fixed Wavelength/ Se-SAD/</td><br />
<td>http://bcsb.lbl.gov/beamlines.htm</td><br />
</b></tr><br />
<tr><br />
<td>ALS </td><br />
<td>5.0.2 </td><br />
<td>Mail & Remote (PRT members) </td><br />
<td>ALS puck </td><br />
<td>MAD </td><br />
<td>http://bcsb.lbl.gov/beamlines.htm </td><br />
</tr><br />
<tr><br />
<td>ALS</td><br />
<td>5.0.3 </td><br />
<td>Mail & Remote (PRT members) </td><br />
<td>ALS puck </td><br />
<td> ? </td><br />
<td>http://bcsb.lbl.gov/beamlines.htm </td><br />
</tr><br />
<tr><br />
<td>ALS</td><br />
<td>4.2.2 </td><br />
<td>Remote capabilities </td><br />
<td>ALS puck ? </td><br />
<td> ? </td><br />
<td>http://bcsb.lbl.gov/beamlines.htm </td><br />
</tr><br />
<br />
<tr><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
<td> </td><br />
</tr><br />
<br />
<br />
</table><br />
<br />
'''''Please copy and paste the empty row for the next person to fill in'''''</div>Jboschhttps://wiki.uni-konstanz.de/ccp4/index.php?title=Synchrotrons&diff=1029Synchrotrons2008-07-24T05:58:24Z<p>Jbosch: </p>
<hr />
<div>For a list of synchrotrons please see [http://www.esrf.eu/UsersAndScience/Links/Synchrotrons Synchrotron beamlines]<br />
<br />
The following beamlines offer mail-in service or remote access:<br />
(compiled from the CCP4 BB July 2008)<br />
<br />
1. The ALS BCSB beamlines http://bcsb.lbl.gov/beamlines.htm have a mail-in type program name collaborative crystallography on beamlines 5.0.2 (MAD) and 5.0.1 (Se-SAD/ Fixed<br />
Wavelength).<br />
Remote access is currently available on the sector 5 stations (5.0.1<br />
5.0.2 and 5.0.3) for PRT members only. ALS 4.2.2 beamline has remote capability<br />
<br />
2. Beamline 3BM1 at the Australian Synchrotron http://www.synchrotron.org.au has full remote access available for users. We use the same Blu-Ice gui as SSRL and use the SAM robot.<br />
When 3ID is available for general user program (2009) it will also have full remote access capabilities.<br />
<br />
3. BNL / NSLS Mail-in program http://www.px.nsls.bnl.gov/, where three scientists spend about one full-time equivalent of effort, employing about one full-time beamline among the six PXRR beamlines. <br />
Access to Beam Time http://www.px.nsls.bnl.gov/beamtime_requests.html Mail-in Program http://www.px.nsls.bnl.gov/Mailin.html<br />
<br />
4. The canadian light source http://www.lightsource.ca/ has at least mail in protein crystallography. <br />
<br />
5. MacCHESS http://www.macchess.cornell.edu/ does "mail-in" collection on all PX beamlines.<br />
<br />
6. All the ESRF MX/PX beamlines (ID14-1, ID14-2, ID14-4, ID23-1, ID29), and the CRG beam-line BM14, have remote access.<br />
http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access<br />
For industrial users there is also the MXpress mail in service:<br />
http://www.esrf.eu/Industry/our_services/xpress-service/xpress-service<br />
<br />
7. SEC-CAT 22-ID and 22-BM at APS http://www.ser-cat.org/ will do both mail-in and remote<br />
<br />
8. SPring8 in Japan http://www.spring8.or.jp/en/about_us/organization/research_utilization/biology has a mail-in system.<br />
<br />
9. SSRL http://smb.slac.stanford.edu/ all PX beamlines have [http://smb.slac.stanford.edu/public/facilities/remote_access/ remote capability]. About 75% of the users use fully remote data collection routinely<br />
<br />
10. CAMD's PX beamline http://www.camd.lsu.edu/gcpcc/GCPCChome.htm has a mail-in program for both consortium members and outside users.<br />
<br />
11. BESSY BL14.1 and BL14.2 http://www.mx.bessy.de, The BESSY-MX group is offering the "mail-in" service for industrial collaborators.<br />
<br />
12. SLS (http://sls.web.psi.ch/): For industrial users there is a Mail-In Service available since more than 3 years. For technical questions please contact Joachim Diez (Joachim.Diez@psi.ch) or Clemens Schulze-Briese (clemens.schulze@psi.ch), for questions about contracts please contact Philipp Dietrich (philipp.dietrich@psi.ch). Remote operation is in preparation for all beamlines. <br />
<br />
A useful link from PDB containing synchrotron info:<br />
http://biosync.rcsb.org/<br />
<br />
<br />
<table border = "1"><br />
<tr><br />
<b><br />
<td>Synchrotron</td><br />
<td>Beamline</td><br />
<td>Mail-in/ Remote</td><br />
<td>type of puck</td><br />
<td>Fixed Wavelength/ Se-SAD/ MAD</td><br />
<td>URL</td><br />
</b></tr><br />
<td>ALS</td><br />
<td>5.0.1</td><br />
<td>Mail-in/ Remote</td><br />
<td>type of puck</td><br />
<td>Fixed Wavelength/ Se-SAD/</td><br />
<td>http://bcsb.lbl.gov/beamlines.htm</td><br />
</b></tr></div>Jboschhttps://wiki.uni-konstanz.de/ccp4/index.php?title=Synchrotrons&diff=1028Synchrotrons2008-07-24T05:55:52Z<p>Jbosch: </p>
<hr />
<div>For a list of synchrotrons please see [http://www.esrf.eu/UsersAndScience/Links/Synchrotrons Synchrotron beamlines]<br />
<br />
The following beamlines offer mail-in service or remote access:<br />
(compiled from the CCP4 BB July 2008)<br />
<br />
1. The ALS BCSB beamlines http://bcsb.lbl.gov/beamlines.htm have a mail-in type program name collaborative crystallography on beamlines 5.0.2 (MAD) and 5.0.1 (Se-SAD/ Fixed<br />
Wavelength).<br />
Remote access is currently available on the sector 5 stations (5.0.1<br />
5.0.2 and 5.0.3) for PRT members only. ALS 4.2.2 beamline has remote capability<br />
<br />
2. Beamline 3BM1 at the Australian Synchrotron http://www.synchrotron.org.au has full remote access available for users. We use the same Blu-Ice gui as SSRL and use the SAM robot.<br />
When 3ID is available for general user program (2009) it will also have full remote access capabilities.<br />
<br />
3. BNL / NSLS Mail-in program http://www.px.nsls.bnl.gov/, where three scientists spend about one full-time equivalent of effort, employing about one full-time beamline among the six PXRR beamlines. <br />
Access to Beam Time http://www.px.nsls.bnl.gov/beamtime_requests.html Mail-in Program http://www.px.nsls.bnl.gov/Mailin.html<br />
<br />
4. The canadian light source http://www.lightsource.ca/ has at least mail in protein crystallography. <br />
<br />
5. MacCHESS http://www.macchess.cornell.edu/ does "mail-in" collection on all PX beamlines.<br />
<br />
6. All the ESRF MX/PX beamlines (ID14-1, ID14-2, ID14-4, ID23-1, ID29), and the CRG beam-line BM14, have remote access.<br />
http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/remote-access<br />
For industrial users there is also the MXpress mail in service:<br />
http://www.esrf.eu/Industry/our_services/xpress-service/xpress-service<br />
<br />
7. SEC-CAT 22-ID and 22-BM at APS http://www.ser-cat.org/ will do both mail-in and remote<br />
<br />
8. SPring8 in Japan http://www.spring8.or.jp/en/about_us/organization/research_utilization/biology has a mail-in system.<br />
<br />
9. SSRL http://smb.slac.stanford.edu/ all PX beamlines have [http://smb.slac.stanford.edu/public/facilities/remote_access/ remote capability]. About 75% of the users use fully remote data collection routinely<br />
<br />
10. CAMD's PX beamline http://www.camd.lsu.edu/gcpcc/GCPCChome.htm has a mail-in program for both consortium members and outside users.<br />
<br />
11. BESSY BL14.1 and BL14.2 http://www.mx.bessy.de, The BESSY-MX group is offering the "mail-in" service for industrial collaborators.<br />
<br />
12. SLS (http://sls.web.psi.ch/): For industrial users there is a Mail-In Service available since more than 3 years. For technical questions please contact Joachim Diez (Joachim.Diez@psi.ch) or Clemens Schulze-Briese (clemens.schulze@psi.ch), for questions about contracts please contact Philipp Dietrich (philipp.dietrich@psi.ch). Remote operation is in preparation for all beamlines. <br />
<br />
A useful link from PDB containing synchrotron info:<br />
http://biosync.rcsb.org/<br />
<br />
<br />
<table border = "1"><br />
<tr><br />
<b><br />
<td>Synchrotron</td><br />
<td>Beamline</td><br />
<td>Mail-in/ Remote</td><br />
<td>type of puck</td><br />
<td>Fixed Wavelength/ Se-SAD/ MAD</td><br />
<td>URL</td><br />
</b></tr></div>Jboschhttps://wiki.uni-konstanz.de/ccp4/index.php?title=Expression_procedure&diff=849Expression procedure2008-05-05T18:04:26Z<p>Jbosch: New page: This is a suggestion given on the ccp4bb May 1st 2008: What I usually do when I have a new construct is I run a cheap experiment with 2 x 50 ml TB cultures and measure the OD over time e...</p>
<hr />
<div>This is a suggestion given on the ccp4bb May 1st 2008:<br />
<br />
<br />
What I usually do when I have a new construct is I run a cheap experiment with 2 x 50 ml TB cultures and measure the OD over time every hour. One culture is induced the other one not, at the end I compare the plots and deduce when it is best to induce and harvest my cells. The things I look at is what is the peak OD of the non-induced and induced culture, when does the non-induced culture reach halve height of their maximum (15 minutes before that timepoint is when I would induce them the next time, of course the aeration varies with volume , +/- antifoam, +/- baffeled flasks). It is not uncommon to see in the induced experiment that the maximum OD drops off at the end, if that is the case better harvest your cells at the peak as they start lysing.<br />
If your expression level is low that can have many factors and I won't go into that at this point.<br />
So if you don't have a growth curve make one, the longer you culture your cells the more your protein might be exposed to proteases that's another thing to consider when being lazy and growing over night cultures, know your system and act accordingly.<br />
<br />
(I'll try to rephrase this in the near future and add some graphics. JB)</div>Jboschhttps://wiki.uni-konstanz.de/ccp4/index.php?title=Expression_and_Purification&diff=848Expression and Purification2008-05-05T18:01:09Z<p>Jbosch: </p>
<hr />
<div>* [[SLIC cloning]]<br />
* [[Tags]]<br />
* [[Expression systems]]<br />
* [[Expression screening]]<br />
* [[Expression procedure]]<br />
* [[Purification]]<br />
* [[Cleavage: wanted and unwanted]]<br />
* [[DNA and DNA-binding proteins]]<br />
* [[Expression and Purification: Tips and Tricks]]<br />
* [[Expression of SeMet labeled proteins]]<br />
* [[Thiols and disulfides]]</div>Jbosch